Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,336,7480CA55.6% 8.2 / 12.6 9D301Y (GAT→TAT) yfaLAIDA‑I family autotransporter adhesin YfaL/EhaC
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (0/5);  total (2/7)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGCAATAGAGTCAACAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATC  >  NZ_CP009273/2336680‑2336808
                                                                    |                                                            
aGCAATAGAGTCAACAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGc                                          <  1:299808/89‑1 (MQ=255)
aGCAATAGAGTCAACAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGc                                          >  2:299808/1‑89 (MQ=255)
    aTAGAGTCAACAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCgaga                                     <  1:118866/90‑1 (MQ=255)
              cAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGc                           >  2:314066/1‑90 (MQ=255)
                               gTATTCCCAATAACCAGCTTTTTCCCGTCGGAAATATAAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGaggaa          <  2:62592/90‑1 (MQ=255)
                                    gcaaaTACCCAGCTTTTTCCCGTCGGCAATATAAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCCCCCGCCGCAGAGGAAGGAcc     <  2:250928/87‑1 (MQ=255)
                                     ccAATACCCAGCTTTTTCCCGCCGGCAATATAAAAGGTAACTTCACTAAAGCCGAGATAAAAAAACCCCCCCGCCGCAGAGGAAGGACCa    <  1:314066/90‑1 (MQ=255)
                                     caaaTACCCAGCTTTTTCCCGCCGGAAATAAAAAAGGAAACTTCACTAAACCCGAAATAAAAAAACCCCCCCGCCGCAGAGGAAGGACCa    <  1:38540/88‑1 (MQ=255)
                                       aaTACCCAGCTTTTTCCCGTCGGCAATAAAAAAGGTAACTTCACTTAACCCGAGATACAAAAACCCCCCCGCCGCAGAGGAAGGACCAtc  <  1:16700/90‑1 (MQ=255)
                                                                    |                                                            
AGCAATAGAGTCAACAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATC  >  NZ_CP009273/2336680‑2336808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑