| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 653,132 | 0 | A | C | 54.5% | 6.4 / 10.3 | 11 | V88G (GTG→GGG) | crcB | fluoride efflux transporter CrcB |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base C (0/6); total (2/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATG > NZ_CP009273/653046‑653215 | ccAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAcacca > 1:552799/1‑90 (MQ=255) cAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAcaccac > 1:80690/1‑90 (MQ=255) cccAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAg < 1:208243/90‑1 (MQ=255) cccAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAg < 1:239150/90‑1 (MQ=255) cccAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAg < 2:543783/90‑1 (MQ=255) cAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATcc > 2:514495/1‑90 (MQ=255) cAGTAATCCCCAGCAAAAGCGGCCCTCTTGTAAAAAAACCCCCCCTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATcc < 1:629849/90‑1 (MQ=255) agccccAGCCAAAGCGGCCTTCTTAAAAAAAAACCCCCACTCCGGCCAAAAATGTTGAGAAGGTTTTTAGACCGCCACAAAATCCggtgg < 2:552799/87‑1 (MQ=255) cAAAGGGGCCTTTTTGAAAAAAAACCCCCCCTTCTGCCAAAAATGTTGAGAAGGTTTTTAAACCGCCACAAAATCCGGTGGTGATTAATa < 2:482240/90‑1 (MQ=255) cAAAGCGGCCCTCTTTAAAAAAAAACCCCCCTTCTGCCAAAAATGTTGAAAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATa < 1:484066/90‑1 (MQ=255) aaaggCGGCCCTCTTGAAAAAAAACCCCCACTTCTGCAAAAAATGTTGAGAAGGTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATa < 2:80690/86‑1 (MQ=255) gccccTTTTGAAAAAAAACCCCCCCTTCTCCCAAAAATGTTGAGAAGTTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTcc < 1:292875/88‑1 (MQ=255) aaaaaCACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATg > 1:14931/1‑90 (MQ=255) | CCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATG > NZ_CP009273/653046‑653215 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |