| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 711,081 | 0 | A | C | 53.3% | 9.3 / 13.9 | 15 | T72P (ACC→CCC) | BW25113_RS03560 | lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (6/1); new base C (0/8); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCC‑GGTGTAAGACAAATT‑GCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAA > NZ_CP009273/710992‑711165 | aaaCCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCC‑GGTGTAAGACAAATT‑GCCGTGGTGATAACTTACCTGataata > 1:469101/1‑90 (MQ=255) gagGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCC‑GGTGTAAGACAAATT‑GCCGTGGTGATAACTTACCTGATAATACccgcc > 1:276584/1‑90 (MQ=255) aggggTAGCTTTGCGGAAAATATTGC‑CCTTTCCGGGTGAAAAAAAAATT‑GCCGTGGTGATAACTTCCCTGATAATCCCCGCCGCTGGCAg < 1:451372/88‑1 (MQ=255) gCATTGCGGTAAATATTGCACCTTTCC‑GGTGTAAGACAAATT‑GCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGGTGTCAg > 1:437221/1‑90 (MQ=255) tatTCCCCCTTTCC‑GGGGAAAGCAAAATT‑CGCGGGGTAACAACTTCCCTAATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGaaaa < 2:27452/90‑1 (MQ=255) ttGCACCTTTCC‑GGTGTAAGACAAATT‑GCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAg > 1:723107/1‑90 (MQ=255) tGCACCTTTCC‑GGTGTAAGACAAATT‑GCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGa > 2:60153/1‑90 (MQ=255) cTTTCCGGGTGTAAGAAAAATT‑GCGGGGGTAAAAACTTACCTAAAAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAactt < 2:280574/90‑1 (MQ=255) tttCC‑GGTGTAAGACAAATT‑GCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAacttac > 2:374120/1‑90 (MQ=255) cc‑GGTGTAAGAAAAATT‑GCCGTGGTGAAAACTTCCCTAATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAActtactta < 2:276584/90‑1 (MQ=255) c‑GGTGAAAGACAAATT‑GCGGGGGGAAAAACTAACCTAATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTAt < 2:519294/90‑1 (MQ=255) ggTGTAAGACAACTT‑GCCGTGGTAATAACTTCCCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATc < 1:665738/90‑1 (MQ=255) gtgaAAGAAAAATG‑GCCGTGGTGATAACTTACCTAAAAACCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCt < 1:438085/90‑1 (MQ=255) aaGACAAATT‑GCCGTGGTGATAACTTACCTGATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCt < 1:55348/86‑1 (MQ=255) aaGACAAATT‑GCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCt > 2:55348/1‑86 (MQ=255) aaaaTTGGCCGTGGTAATAATTTCCCTGATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCataat < 1:558437/89‑1 (MQ=255) ataataCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTaa > 1:133401/1‑90 (MQ=255) | AAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCC‑GGTGTAAGACAAATT‑GCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAA > NZ_CP009273/710992‑711165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |