| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 765,670 | 0 | G | T | 53.8% | 7.0 / 14.6 | 13 | S746I (AGT→ATT) | mngB | mannosylglycerate hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base T (0/7); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCAT > NZ_CP009273/765583‑765756 | tAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGtt > 2:647266/1‑90 (MQ=255) tCGGAAATTAAAATGCCATCCCCGGACCCACAACTCCGTGGTCTGTTTTCTTGCCGCTTAATTTTATTGATTTATCCCGGTACGCCAccc < 2:403677/90‑1 (MQ=255) cGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAgg > 2:60369/1‑90 (MQ=255) aaCTCCGTGGTCTTCTTTCTTGTCCCCTAATTTTATTGATTTATACCGGTCCGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCAt < 1:104627/90‑1 (MQ=255) ggTGGTCTGTTTTCTTGCCGCCAAGTTTTTTTGATTTATCCCGGCCCCCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 2:301170/89‑1 (MQ=255) cggggTCTTTTTTCTTGCGGCCTATGTTTTTTGATTTATCCCGGTCCCCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 2:401511/87‑1 (MQ=255) cGTGGTCTGTTTTCTTGTCGCCTAAGTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 1:74894/90‑1 (MQ=255) gTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTc > 2:786518/1‑90 (MQ=255) ttccTTGTCGCAAAGGTTTTTTGATTTGTCCGGGTACGCAAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTAc < 1:550442/87‑1 (MQ=255) ttCTTGTCGCCTAATTTTTTTGAGTTATCCCGGTCCGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACa < 1:647266/90‑1 (MQ=255) cGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTac > 2:585457/1‑90 (MQ=255) ttttATTGATTTATCCCGGTACCCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACaaaa < 1:538252/89‑1 (MQ=255) tGATTTATCCCGGTCCCCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCAt < 1:60369/90‑1 (MQ=255) | TAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCAT > NZ_CP009273/765583‑765756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |