| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 807,177 | 0 | T | G | 53.3% | 8.6 / 14.3 | 16 | V67G (GTG→GGG) | bioC | malonyl‑ACP O‑methyltransferase BioC |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (8/0); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGA > NZ_CP009273/807090‑807255 | ttCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGa < 1:458361/90‑1 (MQ=255) ccccACGTCCTGGACGCGGGTTGGGGCCCGGGTTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTcg < 1:627434/89‑1 (MQ=255) ccACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCCCGCGCAGGGGGCGGGCCTAGATCTCTcgcc > 2:256967/1‑90 (MQ=255) aCGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAAAGGCACGCGCCGGGGACGGGCCTTGATCTCTccccgc > 2:158562/1‑90 (MQ=255) ggACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCCGGGGACGGCCTTAGATCTCTCGCCGCCCATGCt > 1:696088/1‑90 (MQ=255) aCGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCCTAGATCTCTCGCCGCCAATGCTTg > 1:305199/1‑90 (MQ=255) gggTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAAAGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGttta > 1:343382/1‑88 (MQ=255) ttGTGGACCTGCCGGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGc < 2:573194/90‑1 (MQ=255) gtgGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGGTTGTTTAGGGAc > 1:263732/1‑90 (MQ=255) tggctggATGAGCCGCCACTGGCGGGGACGGCGCGCGCAAGCGACTGCCCTAGAATTCTCCCCGCCTATGCTTGTTTATGCACGCCCGaa > 1:755137/1‑90 (MQ=255) ggctggATGAGCCGCCACTGGCGGGAACGGCACGCCCAGGGGACGGCCCTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCCGAAg > 1:4772/1‑90 (MQ=255) tggATGGGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGAt < 2:43871/90‑1 (MQ=255) gAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGc < 2:580431/90‑1 (MQ=255) ggCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGGGTGTTCAGGCACGCCCGAAGGATGCCGCAGACCATTAtt > 2:255958/1‑89 (MQ=255) gTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCgtgcc < 1:256967/90‑5 (MQ=255) tCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGgaga < 1:571841/90‑1 (MQ=255) | TTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGA > NZ_CP009273/807090‑807255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |