| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,548,257 | 0 | T | G | 52.9% | 15.3 / 17.7 | 17 | Y557S (TAC→TCC) | maeA | malate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (9/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.24e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCG > NZ_CP009273/1548169‑1548338 | gATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGGCCCGGTa > 1:397824/1‑90 (MQ=255) aTGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAt < 1:440704/90‑1 (MQ=255) gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGGCGCGGGATTTgg > 2:589229/1‑90 (MQ=255) gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGGCGCGGGATTTgg > 1:641728/1‑90 (MQ=255) gAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGTGGCGGGATTCGGCTTGc > 2:140629/1‑90 (MQ=255) gagGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAt < 1:21734/90‑1 (MQ=255) gagGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCCGAAATTAt > 1:35317/1‑90 (MQ=255) gagGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGTCGCGGGATTTGGGTTTCCAGGAAatat > 2:376958/1‑90 (MQ=255) cccGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGcc < 2:401368/90‑1 (MQ=255) gCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCa < 2:642384/90‑1 (MQ=255) aCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCgg > 1:676829/1‑90 (MQ=255) ccGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCTGGGCTTCGGc < 2:641728/90‑1 (MQ=255) ggCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTTGGCTTGCCCGAAATTATCGTCAATGGCCTGTTGCAGGGGTTCGGCAGAGGgttt > 1:524085/1‑90 (MQ=255) cTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGTCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCa < 1:410486/90‑1 (MQ=255) cTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCa < 1:123098/90‑1 (MQ=255) ggTAGTCGCGGGATTCGGCTTGCCAGAAAATATCGTCAATGGCCCGTTGCAGGGGTTCGGCAGAGGGTTTTACCCCCCCGCCTtgctgct > 2:370310/1‑90 (MQ=255) aGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTCACCCCCCCGCCTTTCTGCTgcg > 1:354236/1‑90 (MQ=255) | GATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCG > NZ_CP009273/1548169‑1548338 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |