| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,334,242 | 0 | T | C | 62.5% | ‑0.6 / 11.6 | 16 | Q1136R (CAG→CGG) | yfaL | AIDA‑I family autotransporter adhesin YfaL/EhaC |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base C (10/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCCGAC > NZ_CP009273/2334160‑2334331 | cAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAAt < 1:580528/90‑1 (MQ=255) aGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAAAAATCCTGCTGCACGCCCCGATaaaac > 1:601858/1‑88 (MQ=255) gAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGGAAAAACATCCCGCTGCACCCCCCGATAAAACAcctgcgcct > 2:274146/1‑90 (MQ=255) tcaCCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCCGCTGCCCCCCCCGGTAAAACACCCCCGCCCGCggggca > 2:288700/1‑86 (MQ=255) cTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGGAAAAACATCCCCCCGCACCCCCCGATAAAACACCCGCGCCTCGGGTTCAATaac > 2:558008/1‑90 (MQ=255) tGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAAACATCCTGCTGCACCCCCCGATAAAACACCCGCGCCTGCGGGTCAATcacc > 1:41048/1‑90 (MQ=255) tgtgACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCCCATAAATAACCTGCCCCCGCGGTTCAATcaccacacca > 2:762863/1‑90 (MQ=255) gtgACACGCGCGCACGGTTAGCGGCGGGAAAAACATCCTCCTGCACCCCCCGATAAAACACCCGCGCCTGCGGGTCAATCaccacaccac > 2:59768/1‑90 (MQ=255) gtgACACGCGCGCACGGTTAGCGGCGGGAAAAACATCCCGCTGCGCGCCCCGATAAAAAACCCCCGCCCGCGGGTCAATCaccacaccac > 2:59754/1‑90 (MQ=255) cacGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGccc < 2:109901/90‑1 (MQ=255) cgcACGGTTAGCGGCGGTAAAAACATCCTGCTGCACGCCCCGGTAAAACACCCGCGCCCGCGGGTCAATCACCACACCACCCCCCCGTaa > 2:763818/1‑90 (MQ=255) cgcACGGTTAGCGGCGGGAAAAACATCCCTCGGCACGCCCTTCTAAAAAACCCCCGCCCGCGGGTCCATCACCACACCCCCCCCCGGCaa > 2:761182/1‑90 (MQ=255) cACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAAcc < 1:403158/90‑1 (MQ=255) cggTAAAATCATCCTGCTGCCCGCCCCGGTAAATCACCCGCGCCCGCGGGTCAAACACCACACCCCCCCCCGGTAACCCCTAGTACCCCg > 2:162319/1‑90 (MQ=255) atcCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGa < 1:7721/90‑1 (MQ=255) cACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAAt < 1:233872/90‑1 (MQ=255) tGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCcgac > 2:343514/1‑90 (MQ=255) tGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCcgac > 2:69030/1‑90 (MQ=255) | CAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCCGAC > NZ_CP009273/2334160‑2334331 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |