| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,254,020 | 0 | A | G | 57.9% | 8.8 / 16.1 | 19 | I47T (ATC→ACC) | qorA | quinone oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (3/5); new base G (11/0); total (14/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.82e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCGATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGA > NZ_CP009273/4253938‑4254098 | acTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGGAAAAGGCCCTGCGGGTATAtgt > 2:423970/1‑90 (MQ=255)acTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGGAAAAGCCGCTGCGGgtatattt > 2:243102/1‑88 (MQ=255) cGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGGAAAGGCCGCTGCGGATATATGTGTCgg > 2:502760/1‑89 (MQ=255) cTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGGAAAAGCCGCCGCGGGTGTATGTGGCGAtga > 2:313302/1‑88 (MQ=255) ttACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGGAAAGGCCGGTGCGGGTTGATGTTTGGGTaaaa > 2:6837/1‑90 (MQ=255) cGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGGAAAGGCCGCTGCGGGTATATTTGTCGATAAAATTGATGc > 1:451502/1‑90 (MQ=255) gCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCGATAAAATTGATGCCGAt < 1:606663/90‑1 (MQ=255) tCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGGAAAAGCCCCTGCGGGTATATTTGTCGATAAAATTTATGCCGATGGCTTTAttt > 1:18686/1‑90 (MQ=255) ggTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGGAAAGGCCGCTGCGGGTATATTTGTCGATAAAATTTATTCCGATGGCTTTAttttt > 1:585311/1‑89 (MQ=255) ccTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCGATAAAATTGATGCCGATGGCTTTATTTTCGAcc < 2:716272/90‑1 (MQ=255) aTCCGCTGGGTAGCGATGGCGGCGGGTAAAAGCCGCTGCGGGTATATGTGTCGATAAAATTGATGCCGATGGCTTTTTTTTTGACCTggg > 2:185001/1‑89 (MQ=255) aTCCGCTGGGTAGCGATGGCGGCGGGTAAAAGCCGCTGCGGATATATGTGTCGATAAAATTTATGCCGGGGGGTTTATTTTCGACCTggg > 1:656272/1‑89 (MQ=255) cTGGGTAGCGATGGCGGCGGGGAAAGGCCGCTGCGGGTATATGTGTCGGTAAAATTGATGCCGGTGGCTTTTTTTTCGACCTGGGTTTCa > 2:55333/1‑90 (MQ=255) tGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGGTATATGTGTCGATAAAATTGATGCCGATGGCTTTATTTTTGACCTGGATTTCAt > 1:90112/1‑90 (MQ=255) ggTAGAGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCGATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATtc < 1:201880/90‑1 (MQ=255) aTGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCGATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTccgccg < 1:92144/90‑1 (MQ=255) ggcggcGGGTAAAGGCCGCTGCGGGTATATGTGGCGGTAAAATTGATGCCGGTGGCTTTATTTTTGACCCGGGTTTCATTTTTcggcggg > 2:479218/1‑89 (MQ=255) ggcggcGGGGAAAGGCCGCTGCGGGTATATTTGTCGATAAAATTGATGCCGGTGGGTTTTTTTTTGGCCTGGGTTTTATTCTCCGCCggg > 2:609997/1‑89 (MQ=255) cggGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGc < 1:516894/90‑1 (MQ=255) ggCCGCTGCGGATATATGTGTCGATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGGGa > 2:274141/1‑90 (MQ=255) | ACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCGATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGA > NZ_CP009273/4253938‑4254098 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |