| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 70,068 | 0 | A | C | 54.5% | 14.3 / 25.8 | 22 | V314G (GTA→GGA) | thiP | thiamine/thiamine pyrophosphate ABC transporter permease ThiP |
| Reads supporting (aligned to +/- strand): ref base A (8/2); new base C (0/12); total (8/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.41e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGA > NZ_CP009273/69981‑70157 | accccTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAcc < 1:421637/87‑1 (MQ=255) gccgccAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcg > 2:400762/1‑90 (MQ=255) gccgccAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcg > 1:573789/1‑90 (MQ=255) gccgccAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcg > 1:609013/1‑90 (MQ=255) cgccAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcgac > 2:701667/1‑90 (MQ=255) gcgcAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCa > 1:635852/1‑90 (MQ=255) gTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGgc > 1:533257/1‑90 (MQ=255) ccTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAAcagca > 2:540446/1‑90 (MQ=255) gCCACAGCCCGGGTTGTGCCAGCCCTCCCGGCAACTGGCGTTTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAAcagcagca < 1:719167/90‑1 (MQ=255) cggtttGTGCCACCTCTCCCGGCAACTGGCTTTTTCCCCCACCGACGACCCCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGca < 1:701667/86‑1 (MQ=255) cggtttGGGCCACCCCTCCCGGCAACGGGGGTTTCCCCCCTCCCACCAACACCGCCCGTAACGGTGGCAGCAACAGCAGCAGCGCCAGca < 2:791675/86‑1 (MQ=255) ccGTTTGTCCCAGCCCTCCCGGCACCTGGCAATTTCCCCCACCGACGACCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGca < 1:638629/90‑1 (MQ=255) ccGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGca < 1:655922/90‑1 (MQ=255) ccGGTTGTCCCCGCCCTTCCCGCAACGGGGGTTTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGca < 1:238484/90‑1 (MQ=255) ccGGTTGTCCCAGCCCTCCCGGAACCTGGCGTTTCCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGca < 2:177951/90‑1 (MQ=255) ccGGTTGCCCCACCCCTCCCGGCAACGGGCGTTTCCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGca < 2:142997/90‑1 (MQ=255) gtttGTGCCAGCCCTCCCGGCAACGGGCGATTTCCCCCCCCGACGATCCCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGcaca < 2:641451/88‑1 (MQ=255) ggTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGcaca > 1:42601/1‑90 (MQ=255) ggTTGGGCCAGCCCTCCCGGCACCGGGCGATTTCCCCCACCGACGACCCCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGcaca < 2:609013/90‑1 (MQ=255) cACTCCCGGCAACGGGCGTTTCCCCCCACCGACGACCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCgt < 1:797201/90‑1 (MQ=255) cgttttCCCCCATCGCCGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTa < 1:741984/87‑1 (MQ=255) cgttttCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTa < 1:400762/87‑1 (MQ=255) aCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGa > 1:312119/1‑90 (MQ=255) | ACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGA > NZ_CP009273/69981‑70157 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |