Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,903	T→A	D27E (GAT→GAA)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,903	0	T	A	100.0%	145.7 / NA	45	D27E (GAT→GAA)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (24/21);  total (24/21)

TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGT  >  NZ_CP009273/49820‑49982                                                                                    |                                                                                tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc                                                                           <  2:215246/90‑1 (MQ=255)  cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCt                                                                          <  1:638338/90‑1 (MQ=255)        tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTa                                                                    >  1:102555/1‑90 (MQ=255)        tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTa                                                                    >  1:421313/1‑90 (MQ=255)         cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGTATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTaa                                                                   >  2:647227/1‑90 (MQ=255)          aggcTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAAAGCCATCCCGGGGGAGCTGCCTGGCGAACTCGCCTGGTTTtaa                                                                  >  2:120082/4‑90 (MQ=255)          aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTaaa                                                                  >  1:26377/1‑90 (MQ=255)          aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGAACTCGCCTGGTTTaaa                                                                  >  2:168151/1‑90 (MQ=255)             cTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGc                                                               <  2:26377/90‑1 (MQ=255)               gATTGCGGCTTTAGCGGTCCATGCCCTTGTCGGCAGGGAAACCGCTATTCCGGGGAACCCGCCTGCCGAACCCGCCTGGTTTAAACGCaa                                                             <  2:617972/90‑1 (MQ=255)               gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                             >  2:468104/1‑90 (MQ=255)               gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGGTTAAACGCaa                                                             >  1:381331/1‑90 (MQ=255)                aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAac                                                            <  1:627996/90‑1 (MQ=255)                 ttGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAac                                                            >  2:294302/1‑89 (MQ=255)                 ttGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAac                                                            <  1:294302/89‑1 (MQ=255)                    cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCt                                                        <  2:630069/90‑1 (MQ=255)                    cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCt                                                        <  2:421313/90‑1 (MQ=255)                    cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCt                                                        <  1:142003/90‑1 (MQ=255)                    cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCt                                                        <  1:13929/90‑1 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt                                                       >  1:420088/1‑90 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt                                                       >  2:671970/1‑90 (MQ=255)                         ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTtaaat                                                   >  1:478975/1‑90 (MQ=255)                                tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCg                                            <  1:668850/90‑1 (MQ=255)                                    tCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAAAAAACCCGTGAt                                        >  2:218336/1‑90 (MQ=255)                                     cgcgTTATCGGCATGGAAAACGCCATGGCGGGGGACCTGCCCGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGGGaat                                       >  1:588677/1‑88 (MQ=255)                                      gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                      <  2:421060/90‑1 (MQ=255)                                       cgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGCGGTTAt                                     >  1:722605/1‑90 (MQ=255)                                         ttATCGGCATGAAAACCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAACCCCGTGATTATgg                                   <  2:478894/90‑1 (MQ=255)                                              ggCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                              <  1:772963/90‑1 (MQ=255)                                                cATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa                            >  2:137935/1‑90 (MQ=255)                                                cATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa                            >  1:61242/1‑90 (MQ=255)                                                 aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                           <  1:468104/90‑1 (MQ=255)                                                  tGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTta                                                      >  1:494241/1‑62 (MQ=255)                                                  tGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTta                                                      <  2:494241/62‑1 (MQ=255)                                                        aCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTggg                    >  1:435611/1‑90 (MQ=255)                                                         cGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                   <  1:478300/90‑1 (MQ=255)                                                            cATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                             >  1:652685/1‑45 (MQ=255)                                                            cATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                             <  2:652685/45‑1 (MQ=255)                                                                ccGTGAACCCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc            <  1:590979/90‑1 (MQ=255)                                                                  gTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg          >  1:408297/1‑90 (MQ=255)                                                                  gTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg          >  2:703821/1‑90 (MQ=255)                                                                   tGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgt         >  2:143909/1‑90 (MQ=255)                                                                    ggAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc        <  1:671970/90‑1 (MQ=255)                                                                         ctgcctgcCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg   >  1:79412/1‑90 (MQ=255)                                                                          tgcctgccGAACTCGCCTGGTTTAAACGCAACACCTTAAATAACCCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt  <  2:403243/90‑1 (MQ=255)                                                                                    |                                                                                TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGT  >  NZ_CP009273/49820‑49982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑