| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,184,613 | A→T | I207N (ATC→AAC) | phoQ ← | two‑component system sensor histidine kinase PhoQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,184,613 | 0 | A | T | 95.2% | 67.0 / ‑3.4 | 21 | I207N (ATC→AAC) | phoQ | two‑component system sensor histidine kinase PhoQ |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (7/13); total (7/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.62e-01 | |||||||||||
ATGTTCTTCCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGATCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTCCACCGGAATGGTATCC > NZ_CP009273/1184531‑1184699 | aTGTTCTTCCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTaa > 1:431146/1‑90 (MQ=255) gTTCTTCCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAAca < 2:480146/90‑1 (MQ=255) gTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTgg > 1:83851/1‑90 (MQ=255) ggACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTgaga < 1:689258/90‑1 (MQ=255) ggACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTgaga < 2:361994/90‑1 (MQ=255) gACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGagag < 1:279230/90‑1 (MQ=255) tcttTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGcaca < 1:267717/90‑1 (MQ=255) tcttTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGATCACTAACAGCAGATTGGCTGAGAGcaca < 1:702/90‑1 (MQ=255) cAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATaaa > 1:34560/1‑90 (MQ=255) gggCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAAcc < 2:34560/90‑1 (MQ=255) cGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGcac < 2:209567/73‑1 (MQ=255) cGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGcac > 1:209567/1‑73 (MQ=255) aTGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAg < 2:575599/90‑1 (MQ=255) gggCGTAAACTCCACCAGGCGGCGACCCACAGCATCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGAcc < 1:556249/90‑1 (MQ=255) gggCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGAcc > 1:434723/1‑90 (MQ=255) gggCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGAcc > 1:133563/1‑90 (MQ=255) aGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTa > 2:698453/1‑90 (MQ=255) gcgACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTcc < 1:698453/90‑1 (MQ=255) gcgACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTcc < 1:278288/90‑1 (MQ=255) cagcagCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTCCACCGGAAt < 2:133563/90‑1 (MQ=255) gggTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTCCACCGGAATGGTATcc < 2:83851/90‑1 (MQ=255) | ATGTTCTTCCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGATCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTCCACCGGAATGGTATCC > NZ_CP009273/1184531‑1184699 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |