Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,902,932	T→C	D31G (GAT→GGT)	mgrB ←	PhoP/PhoQ regulator MgrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,902,932	0	T	C	100.0%	96.0 / NA	26	D31G (GAT→GGT)	mgrB	PhoP/PhoQ regulator MgrB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/10);  total (16/10)

GGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGA  >  NZ_CP009273/1902850‑1903004                                                                                   |                                                                         ggAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATc                                                                   >  1:499941/1‑90 (MQ=255)  gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCg                                                                  <  2:290934/90‑1 (MQ=255)      aaaaTCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGcaca                                                              >  1:702266/1‑90 (MQ=255)       aaaTCTAGTGCTGAAAAAATGATATCACCACGGGATAGACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCAcat                                                             <  1:193440/90‑1 (MQ=255)          tCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCAcatcat                                                          <  2:765698/90‑1 (MQ=255)          tCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCAcatcat                                                          <  1:757666/90‑1 (MQ=255)              gTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTg                                                      >  2:8873/1‑90 (MQ=255)              gTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTg                                                      >  2:484085/1‑90 (MQ=255)                   gAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAATAc                                                 >  1:139436/1‑90 (MQ=255)                   gAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAATAc                                                 >  2:264219/1‑90 (MQ=255)                   gAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAATAc                                                 >  1:675875/1‑90 (MQ=255)                                   cacGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACCCCCTGATCGCACATCATGTTGAATACCTGCGCCCAAagcagc                                 <  1:445362/90‑1 (MQ=255)                                    acGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAgcagca                                >  2:744394/1‑90 (MQ=255)                                     cGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaa                               <  2:236556/90‑1 (MQ=255)                                       ggATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACCCCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagc                             <  2:95278/90‑1 (MQ=255)                                       ggATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagc                             <  2:72314/90‑1 (MQ=255)                                         aTAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAgcaagcaa                           >  2:590539/1‑90 (MQ=255)                                          tAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                          >  1:425609/1‑90 (MQ=255)                                            aaCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGcc                        >  2:25150/1‑90 (MQ=255)                                               tGGTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAac                     >  2:704084/1‑90 (MQ=255)                                                 gTTAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAacac                   <  2:37892/90‑1 (MQ=255)                                                  ttAATGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacc                  >  2:754928/1‑90 (MQ=255)                                                      tGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAAt                                                   <  2:130669/53‑1 (MQ=255)                                                      tGGCACAAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAAt                                                   >  1:130669/1‑53 (MQ=255)                                                          acaAATTCCGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAAc          >  1:680766/1‑90 (MQ=255)                                                                  cGCTGAAAAATTGTACACCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGa  >  2:70413/1‑90 (MQ=255)                                                                                   |                                                                         GGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGA  >  NZ_CP009273/1902850‑1903004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑