| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,254,480 | 0 | A | C | 53.3% | 4.7 / 14.2 | 15 | V137G (GTG→GGG) | fruA | PTS fructose transporter subunit IIBC |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base C (0/8); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTCGGGC > NZ_CP009273/2254401‑2254555 | aaTCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGcc > 1:11705/1‑90 (MQ=255) gctgctGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCt > 1:189256/1‑90 (MQ=255) tcGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTcaccca < 2:84814/56‑1 (MQ=255) tcGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTcaccca > 1:84814/1‑56 (MQ=255) gTGATTGCATTCCCCGCGCCAACAGAACCACGGGTTTCAACTTTCCCCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc < 1:185849/90‑1 (MQ=255) gTGATTGCATTCCCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc < 1:58225/90‑1 (MQ=255) cATTCCCCGCGCCAACAGAACCACGGGTTTCAACTTTCCCCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATaa < 1:470822/90‑1 (MQ=255) aTTCCCCGCGCCAACAGAACCCCGGGTTTCAACTTTCCCCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATaaa < 1:40150/90‑1 (MQ=255) cccGCGCCAACAGACCCACGGTTTTCATCTTCCCCCCCCCAGCCATGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGgtg < 2:446498/90‑1 (MQ=255) cccGCGCCAACAGACCCACGGGTTTCAACTTTCCCCCCCCAGCCCCGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGgtg < 1:394831/90‑1 (MQ=255) ccGCGCCAACAAAACCAGGGTTTTCACCTTCCCCCCCCCAGCCCCTTTTTTTCGCTTCGGTTTCAAGGGTTTCAGCCGCCATAAAGgtgt < 2:216503/90‑1 (MQ=255) ccGCCCCAACAAAACCCGGGGTTTCAACTTTCCCCCCCCAGCCACTTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGgtgt < 1:249191/90‑1 (MQ=255) cgcCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGtgtgtg > 2:217753/1‑90 (MQ=255) aCAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACg > 1:216503/1‑90 (MQ=255) aCAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACg > 1:129208/1‑90 (MQ=255) ccccGGTTTTAACCTTCCCCCCCCCAGCCCCGTTTTTTCGCTTCGGTTTCAAGGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTc < 2:31790/87‑1 (MQ=255) gggTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTCGGGc < 1:217753/90‑1 (MQ=255) | AATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTCGGGC > NZ_CP009273/2254401‑2254555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |