| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,032,398 | 0 | A | C | 57.1% | 2.1 / 10.8 | 14 | G235G (GGT→GGG) | xerD | site‑specific tyrosine recombinase XerD |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (0/8); total (4/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCCAGCACAGCATAATGTTTAATACGGTGCCAGAAGGTCTGTCGCGTCATCTGCTGCGCA > NZ_CP009273/3032309‑3032461 | tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATa > 2:431781/1‑90 (MQ=255) ccgcgTAAATCCGCCCCATGATTTAATAAATGAGTGGAAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATCCCCGcc < 1:271607/89‑1 (MQ=255) acGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGcc > 1:208318/1‑90 (MQ=255) tAACTCCGCACCATTTTTTAATAAATGAGGGGAAAAAGCGGGCCGCAACACATGCGGTGACAGTTTTTCGCTGTCGATCCCCGCcagcac < 2:221187/90‑1 (MQ=255) acagtCCGCACCATGATTTAAAAAATGAGTGGAAAAAGCGGGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATCCCCGCcagcac < 2:184755/86‑1 (MQ=255) aaaaCCCGCACCATGATTTAAAAAATGAGTGGCAAAAGCGGGACGCAACACATGGGGTGACAGTTTTTCGCTGTCGATCCCCGCcagcac < 2:208318/89‑1 (MQ=255) ccGCCCCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATCCCCGCCAGCACAGCAt < 1:113499/90‑1 (MQ=255) ccGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCCAGCACAGCAt > 1:148254/1‑90 (MQ=255) ccACCATTTTTAAATAATGGGGTGACAAACGCGTGACACAACACATGGGGTCACACTTTTTCGCGGTCTATCCCCGCCAGCACAGCATaa < 2:201619/89‑1 (MQ=255) gATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCCAGCACAGCATAATGtctgt > 1:184755/1‑86 (MQ=255) tttAAAAAATGAGTGGAAAAACCGTGACGCAACCCATGGGGGGACAGCTTTTCGCTGTCGATACCCGCCAGCACAGCATAATGTTTAATa < 1:210449/90‑1 (MQ=255) ttAAAAAATGAGGGAAAAAAGCGGGCCCCAAAAAATGGGGTAACAGTTTTTCGCTGTCGATCCCCGCCAGCACAGCATAATGTTTAATAc < 2:414954/90‑1 (MQ=255) aaaaaaTGAGTGGAAAAAGCGTGACGCAACACAGGGGGTGACAGCTTTTCGCTGCCGATACCCGCCAGCACAGCATAATGTTTAATACgg < 1:451743/87‑1 (MQ=255) gAGTGGAAAAAGCGTGACGCAACACATGGGGTGACAGTTTTTCGCTGTCGATCCCCGCCAGCACAGCATAATGTTTAATACGGTGCCAGa < 2:280378/90‑1 (MQ=255) tGCGGTGACAGCTTTTCGCTGTCGATACCCGCCAGCACAGCATAATGTTTAATACGGTGCCAGAAGGTCTGTCGCGTCATCTGCTGCGCa > 2:448352/1‑90 (MQ=255) | TGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCCAGCACAGCATAATGTTTAATACGGTGCCAGAAGGTCTGTCGCGTCATCTGCTGCGCA > NZ_CP009273/3032309‑3032461 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |