Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,502,0160AG52.4% 24.7 / 29.9 21F386S (TTC→TCC) yhfWphosphopentomutase
Reads supporting (aligned to +/- strand):  ref base A (0/10);  new base G (11/0);  total (11/10)
Fisher's exact test for biased strand distribution p-value = 2.84e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.82e-05
Rejected as consensus: Frequency below/above cutoff threshold.

AACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCA  >  NZ_CP009273/3501928‑3502103
                                                                                        |                                                                                       
aaCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGaa                                                                                        <  2:266023/90‑1 (MQ=255)
     gTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGgaaaaat                                                                                   >  1:462642/1‑88 (MQ=255)
     gTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGGGGGGgaaaaat                                                                                   >  2:112612/1‑88 (MQ=255)
     gTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGCGGgaaaaat                                                                                   >  1:221304/1‑88 (MQ=255)
     gTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGCGGgaaaaat                                                                                   >  1:276090/1‑88 (MQ=255)
      tCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGCGGGAAAAATc                                                                                  >  1:305709/1‑90 (MQ=255)
        ggATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTcac                                                                                <  2:153763/90‑1 (MQ=255)
                     tAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGcccc                                                                   <  1:112612/90‑1 (MQ=255)
                          tgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAATTCACACACGGTAGCCCCCCCAt                                                              >  1:111926/1‑90 (MQ=255)
                           gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACGGGAGCCCCCCCATc                                                             >  1:56619/1‑90 (MQ=255)
                           gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGGGCGGGAAAAATTACACACGGGAGGCCCCCCATc                                                             >  1:226190/1‑90 (MQ=255)
                           gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGGAGCCCCcccaac                                                             >  1:145665/1‑88 (MQ=255)
                           gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGGGCGGGAAAAATCACAAACCGGAGCCCCcccacc                                                             >  2:376972/1‑88 (MQ=255)
                                   cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCg                                                     <  2:175561/90‑1 (MQ=255)
                                                 gaaagaaaAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACAAGGGAGCCCCCCCCACACGAAACGTGGGGCGCCCAccc                                       >  2:242407/1‑89 (MQ=255)
                                                   aagaaaagaGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATAAAACACAGGAGCCCCCCCCCCAGAAAACGGGGGGGGCCCACCCAg                                     >  2:127048/1‑90 (MQ=255)
                                                    agaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGc                                    <  2:260622/90‑1 (MQ=255)
                                                    agaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGc                                    <  1:127048/90‑1 (MQ=255)
                                                        aagaGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACCGTAGCCCCCCCCACACAAAACGGGGGGGGGGCCCCGAGCTGCg                                >  1:400248/1‑90 (MQ=255)
                                                          gagCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTa                              <  2:350073/90‑1 (MQ=255)
                                                           agCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAg                             <  2:145665/90‑1 (MQ=255)
                                                            gCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACCGGAGCCCCCCCATCAGAAAACGGGGGGGGCACACCCCGCCGCGGAGc                            >  2:48373/1‑90 (MQ=255)
                                                            gCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGc                            <  2:346977/90‑1 (MQ=255)
                                                               aCCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACCGTAGCCCCCCCATCAGAAAACGGGGGGCGCACACCGAGCTGCGTAGCggt                         >  1:314144/1‑88 (MQ=255)
                                                                                      ggAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCa  <  1:104703/90‑1 (MQ=255)
                                                                                      ggAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCa  <  2:314144/90‑1 (MQ=255)
                                                                                        |                                                                                       
AACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCA  >  NZ_CP009273/3501928‑3502103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑