| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,806,632 | 0 | C | T | 50.0% | 0.3 / 12.5 | 22 | S181L (TCG→TTG) | coaBC | bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate‑‑cysteine ligase CoaBC |
| Reads supporting (aligned to +/- strand): ref base C (1/10); new base T (11/0); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTA > NZ_CP009273/3806558‑3806718 | ggCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGGATTTTTTGCCCCGCAaccaccc > 1:437099/1‑89 (MQ=255)ggCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCCTTTTTTTCCCCTCAACAAccc > 1:289140/1‑89 (MQ=255)ggCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAGGGGCTTTTTTTCCCCTCCACGAACt > 2:103417/1‑90 (MQ=255) gtgATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATc < 1:103417/90‑1 (MQ=255) gATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCCTTTTTTTCCCCTCAACGACCCGAAAAATCTg > 1:362254/1‑90 (MQ=255) tatCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGaa < 1:251946/90‑1 (MQ=255) gTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTTGCCCGTCAACGACCTGAAACATCTGAACATTa > 1:421411/1‑90 (MQ=255) gggCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGAtt < 2:421411/90‑1 (MQ=255) aaTGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTTGCCCGTCAACCACCCGAAAAATCTGAACATTATTATTAacgc > 1:363330/1‑90 (MQ=255) cagcGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGa < 1:448219/87‑1 (MQ=255) gTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGAcgcg < 2:229509/90‑1 (MQ=255) ttGTGGATATGGCGGTAGCGCCTTTTTTGCCCCTCCACCACCTGAAACATCTTAACATTATGATTACCCCCCGCCCCACGCGGGGACCGc > 2:137900/1‑90 (MQ=255) ttGTGGATATGGCGGTAGCGCATTTTTTGCCCGTCAACGACCTGAAAAATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAAc > 1:63556/1‑87 (MQ=255) ttGTGGATATGGCGGTAGCGCATTTTTTGCCCGTCAACGACCTGAAAAATCTGAACATTATGATTACCGCCGGCCCCACGCGTGAACCGc > 1:18512/1‑90 (MQ=255) ttGTGGATATGGCGGTAGCGCATTTTTTCCCCCTCAACCACCTGGAAAATCTTGACAATATGATTTCCCCCGGGCCCCCGCGGGaaaccc > 2:77156/1‑86 (MQ=255) ttGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAAc < 2:63556/87‑1 (MQ=255) tatGGCGGTAGCGCATTTTTCGCCCGTCAACGACATGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATcc < 1:139617/90‑1 (MQ=255) tGGCGGTAGCGCATTTTTTGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCCGCCCCACGCGGGGAACCCCCGATCCgg > 2:40029/1‑90 (MQ=255) cgcATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTtat > 1:422033/1‑89 (MQ=255) cgcATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTtat < 2:422033/89‑1 (MQ=255) ccATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTatat < 1:344396/89‑1 (MQ=255) tttCGCCCGTCAACGCCCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTa < 1:76100/90‑1 (MQ=255) | GGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTA > NZ_CP009273/3806558‑3806718 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |