| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,101,675 | 0 | G | T | 60.0% | ‑1.5 / 11.4 | 10 | N156K (AAC→AAA) | yiiQ | YiiQ family protein |
| Reads supporting (aligned to +/- strand): ref base G (4/0); new base T (0/6); total (4/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTG > NZ_CP009273/4101596‑4101760 | cAGCGAGGGCCCCCCCTTTTTCGCCGTTGCCCGCGAAAACAAAACGCGGTCCCCCTTCTGTCTCGGTGTAATAACTTTTTTTTTCCCccg < 2:57679/90‑1 (MQ=255) ggTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtag > 1:440327/1‑90 (MQ=255) gCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCg > 1:114541/1‑90 (MQ=255) ttttCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGc > 2:169406/1‑90 (MQ=255) ttccccGCAAAAAAATAACGCGGTCCCCTTTCTGTCTGGGTGTAATATCTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTc < 2:186245/86‑1 (MQ=255) ttGTCCCCCACAACAAACCGCGGTCCCCCTTCTGTCTCGGTGTAATAATTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTc < 1:244379/90‑1 (MQ=255) tgcccGCAAAAACATACCGCGGTGCCCTTTTTGTCGGGGTTTAATAGGTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCt < 2:33870/87‑1 (MQ=255) taaaacaAACCGCAGTGCTCTTTCTGTTGGGGTTAAATAGTTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtg < 2:424521/87‑1 (MQ=255) cGCAGTCCCCCTTCTGTCTGGGTGTAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTc < 1:169406/90‑1 (MQ=255) gTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTa > 2:462542/1‑90 (MQ=255) gtttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg > 2:402390/1‑90 (MQ=255) gtttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg > 2:435763/1‑90 (MQ=255) | CAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTG > NZ_CP009273/4101596‑4101760 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |