| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,475,282 | 0 | T | G | 57.1% | 16.2 / 19.4 | 21 | T163P (ACC→CCC) | pepA | leucyl aminopeptidase |
| Reads supporting (aligned to +/- strand): ref base T (2/7); new base G (12/0); total (14/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.10e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.98e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTTTGCTGCTTTAATCCCGGCGGCAATCGCCAGACCGTGCTGGATCGCGCGCTCACCGCTGGTCAGTTCACGGCGGGTCGGCACGTTGAACACCATCTTACGCAGCGGACGACGCGGTTCGCTCTTGTTCGTTTTCAGCTGATCGAAACTGTAGA > NZ_CP009273/4475204‑4475359 | cTTTTGCTGCTTTAATCCCGGCGGCAATCGCCAGACCGTGCTGGATCGCGCGCTCACCGCTGGTCAGTTCACGGCGGGGCGGCACGTTGa > 2:215372/1‑90 (MQ=255) ttGCTGCTTTAATCCCGGCGGCAATCGCCAGACCGTGCTGGATCGCGCGCTCACCGCTGGTCAGTTCACGGCGGGTCGGCACGTTGAaca < 1:226651/90‑1 (MQ=255) tgctgcTTTAATCCCGGCGGCAATCGCCAGACCGTGCTGGATCGCGCGCTCACCGCTGGTCAGTTCACGGCGGGTCGGCACGTTGAacac < 1:215372/90‑1 (MQ=255) tgctgcTTTAATCCCGGCGGCAATCGCCAGACCGTGCTGGATCGCGCGCTCACCGCTGGTCAGTTCACGGCGGGTCGGCACGTTGAacac < 2:324079/90‑1 (MQ=255) tgctgcTTTAATCCCGGCGGCAATCGCCAGACCGTGCTGGATCGCGCGCTCACCGCTGGTCAGTTCACGGCGGGTCGGCACGTTGAacac < 2:139832/90‑1 (MQ=255) aTCGCCAGACCGTGCTGGATCGCGCGCTCACCGCTGGTCAGTTCACGGCGGGGCGGCACGTTGAAAACCATCTTACGCAGCGGACGAcgc > 1:146911/1‑90 (MQ=255) ccAGACCGTGCTGGATCGCGCGCTCACCGCTGGTCAGTTCACGGCGGGTCGGCACGTTGAACACCATCTTACGCAGCGGACGACGCGGtt < 1:163863/90‑1 (MQ=255) gTGCTGGATCGCGCGCTCACCGCTGGTCAGTTCACGGCGGGTCGGCACGTTGAAAACCATCTTACGCAGCGGACGACGCGGGTCGCTCtt > 1:380108/1‑90 (MQ=255) gTGCTGGATCGCGCGCTCACCGCTGGTCAGCTCACGGCGGGGCGGCACGTTTAAAACCCTCTTTCGCAGCGGACGACGCGGTTCGctcct > 1:280826/1‑88 (MQ=255) tGCTGGATCGCGCGCTCACCGCTGGTCAGTTCACGGCGGGGCGGCACGTTGAAAACCCCCTTACCCCGCGGGCGACGCGGGTTGCTCttt > 2:102209/1‑89 (MQ=255) gcgcgcTCACCGCTGGTCAGTTCACGGCGGGGCGGGGCGTTGGACACCACCTTACGCAGCGGGCGACGCGGGTCGCTCTTGttttttttt > 2:339252/1‑89 (MQ=255) gcgcgcTCACCGCTGGTCAGTTCACGGCGGGGCGGCGCGTTGAACAACACCTTACTCCGGGGGCGACGCGGGTCGAGCTTTGTCGTGTTc > 2:389543/1‑90 (MQ=255) gcgcgcTCACCGCTGGTCAGTTCACGGCGGGGCGGCGCGGTGAAAACCCTCCTACGCAGCGGGCGACGCGGGTCGCTCCTGTTTGttttt > 2:311860/1‑89 (MQ=255) gcgcgcTCACCGCTGGTCAGTTCACGGCGGGGCGGCCCGTTGAACACCACCTTACGCCGCGGGGGACGCGGGTCGCTctttttctttttt > 2:404222/1‑89 (MQ=255) gcgcgcTCACCGCTGGTCAGTTCACGGCGGGGCGGCACGTTGAACACCCACTTTCGCAGCGGGCGACGGGGGTTGCTCTTGTTTGttttt > 1:416698/1‑89 (MQ=255) gcgcgcTCACCGCTGGTCAGTTCACGGCGGGGCGGCACGTTGAACACCATCTTTCGCAGCGGACGACGCGGTTCGCTCTTTTTCGTTTTc > 1:138003/1‑90 (MQ=255) gcgcgcTCACCGCTGGTCAGTTCACGGCGGGGCGGCACGTTGAACACCAACTTACCCAGCGGGCGACGCGGGTCGCTCTTTTTCGttttt > 1:294753/1‑89 (MQ=255) gcgcgcTCACCGCTGGTCAGTTCACGGCGGGGCGGCACGTTGAAAACCATCTTTCGCAGCGGGCGACGCGGGTCGCTCTTGTTTGttttt > 1:459600/1‑89 (MQ=255) aCCGCTGGTCAGTTCACGGCGGGTCGGCACGTTGAACACCATCTTACGCAGCGGACGACGCGGTTCGCTCTTGTTCGTTTTCAGCTGATc < 1:380065/90‑1 (MQ=255) aCCGCTGGTCAGTTCACGGCGGGTCGGCACGTTGAACACCATCTTACGCAGCGGACGACGCGGTTCGCTCTTGTTCGTTTTCAGCTGATc < 2:459600/90‑1 (MQ=255) gTTCACGGCGGGTCGGCACGTTGAACACCATCTTACGCAGCGGACGACGCGGGTCGCTCTTGTTCGTTTTCAGCTGATCGAAACTGTaga > 1:78405/1‑90 (MQ=255) | CTTTTGCTGCTTTAATCCCGGCGGCAATCGCCAGACCGTGCTGGATCGCGCGCTCACCGCTGGTCAGTTCACGGCGGGTCGGCACGTTGAACACCATCTTACGCAGCGGACGACGCGGTTCGCTCTTGTTCGTTTTCAGCTGATCGAAACTGTAGA > NZ_CP009273/4475204‑4475359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |