Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,527,9260AC54.5% 4.6 / 10.8 11G219G (GGT→GGG) nanMN‑acetylneuraminate epimerase
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAA‑GCCAGG‑TTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAG  >  NZ_CP009273/4527851‑4528001
                                                                             |                                                                           
ggTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAA‑GCCAGG‑TTTTATCACCTTTATTCACAAc                                                               >  2:26180/1‑90 (MQ=255)
 gTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAA‑GCCAGG‑TTTTATCACCTTTATTCACAAcc                                                              >  2:266937/1‑90 (MQ=255)
   gAAATCAATTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAA‑GCCAGG‑TTTTATCCCCTTTATTCACAAccgc                                                            <  1:102847/90‑1 (MQ=255)
             taaaaTCCGGCCCCCTTTCCAAATCCTGGTTGGGCTTCGCATTTAAAAA‑CCCGGT‑TTTTACCCCCTTTATCCACACCCGCCGCACCAGcc                                                  <  1:258558/88‑1 (MQ=255)
                            gTTCGCAATCCTGGTTTGGCTTCGCCATTAATAA‑GCCAGG‑TTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGc                                   >  2:441020/1‑90 (MQ=255)
                                      cttgTTTTGCCTCCCCAATAAAAA‑ACCAGGTTTTTATCCCTTTTATCCCAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCa                          <  1:314892/87‑1 (MQ=255)
                                             ggCTTCGCCATTAATAA‑GCCAGG‑TTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACt                  >  1:431131/1‑90 (MQ=255)
                                             ggCTTCCCCCTTAAAAACGCCGGT‑TTTTACCCCTTTTATCCAAACCCGCCGCCCCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAAc                   <  2:298700/90‑1 (MQ=255)
                                                    cctttAAAAA‑GCCAGT‑TTTTATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGc           <  1:16520/87‑1 (MQ=255)
                                                       ttAATAA‑GCCAGG‑TTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtgc        >  1:298700/1‑89 (MQ=255)
                                                             a‑GCCGGT‑TTTTACCCCTTTTATCCAAAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAg  <  1:144625/90‑1 (MQ=255)
                                                                             |                                                                           
GGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAA‑GCCAGG‑TTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAG  >  NZ_CP009273/4527851‑4528001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑