| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 135,553 | 0 | A | C | 53.3% | 8.3 / 11.4 | 15 | G720G (GGT→GGG) | gcd | quinoprotein glucose dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (4/3); new base C (0/8); total (4/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCT > NZ_CP009273/135474‑135638 | ccTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTgg > 2:362216/1‑90 (MQ=255) tttGCTCTTGTTTTAAGCGCGCGGGTATTTTCCTGCCTGGGCGGCCAAAACCAGCACGTTCCCCGCCGTGGAGATTGGCCCGCCCAGCAt < 2:459379/89‑1 (MQ=255) cTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGc < 2:325514/90‑1 (MQ=255) cATTTTTTAAGCGCGCGGGTATTTATCTGCCGTAGCGGCGAAAAACAGCACGTTCCCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCAt < 2:125465/90‑1 (MQ=255) cATGTTGTAGGCGCGGGGGTAGTTTTCTGCGGTGGGGGGGAAAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCAt < 2:314792/90‑1 (MQ=255) cATGTTGTAGGCGCGCGGGTATTTATCTGCCGTGGCGGCGAAAAACAGCCCGTTCCCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCAt < 2:219092/90‑1 (MQ=255) gttgtAAGCGCGCAGGTAGTTATCTGCCGTGGCGGCAATAAACAGCACGTTCCCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATAcc < 2:6001/90‑1 (MQ=255) gttgtAAGCGCGCAGGTAGTCATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATAcc < 2:134990/90‑1 (MQ=255) ttgaAAGCGCGCGGGTATTTATCTGCCGGGGGGGCGAAAAACAGCCCGTTCCCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATAccc < 2:351069/90‑1 (MQ=255) gcgcAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGaa > 2:27881/1‑90 (MQ=255) tGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCgg > 2:352601/1‑90 (MQ=255) gcggcgATAAACAGCCCGTTCCCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCggg < 2:397399/90‑1 (MQ=255) gcggcgAAAAACAGCCCGTTCCCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCggg < 2:8984/90‑1 (MQ=255) aCGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTcc > 1:440811/1‑90 (MQ=255) cGTTCCCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCt < 1:75391/90‑1 (MQ=255) | CCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCT > NZ_CP009273/135474‑135638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |