| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 673,607 | 0 | C | A | 53.8% | 5.9 / 14.8 | 13 | T246N (ACT→AAT) | djlB | J domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (2/4); new base A (7/0); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAAACTGCACAGAAATTTACTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTTATTATGAATACGCGCAACGCGTCTATTGTGGAGAAGGCGAAA > NZ_CP009273/673518‑673675 | aaaaCTGCACAGAAATTTACTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAAc < 2:377767/90‑1 (MQ=255) ttACTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAAATGAAACTGATAATTaa > 2:383639/1‑90 (MQ=255) aCTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGa < 1:25745/90‑1 (MQ=255) gCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATg > 1:6197/1‑87 (MQ=255) gCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATg < 2:6197/87‑1 (MQ=255) tGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCt < 1:390076/90‑1 (MQ=255) aGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTtattat < 1:224201/90‑1 (MQ=255) gcgcAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTtattatt > 1:199240/1‑89 (MQ=255) ggTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAAATGAACCTGATAATGAAGATGCGCCCTTTTATGAATaa > 1:48007/1‑89 (MQ=255) ggTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAAATGAACCCGATAATGAAGAATCGCCTTATTTTTAATaa > 2:356962/1‑89 (MQ=255) gCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAAATGAACCTGATAATGAAGATGCGCCTTATTATGAATACgcgc > 1:181312/1‑90 (MQ=255) ccGAACTTCTGGATTACGCCCAGTCATGGCGGGGAAATGAACCCGATAATTAAGATTCGCCCTTTTTTTAATAAGCGCAACGCGGCtttt > 2:61883/1‑90 (MQ=255) gAACTTCTGGATTACGCCCAGTCATGGCGGGGAAATGAACCTGATAATGAAGATGCGCCTTTTTTTTAATACGCGCAACGCGTCTATtgt > 2:197360/1‑90 (MQ=255) aCGCCCAGTCATGGCGGGGAAATGAAACTGATAATTAAAATGCGCCCTTTTTTGAATACGCGCCACACGGCTTTTTTGGGGGAGGGGaaa > 2:39676/1‑90 (MQ=255) | AAAACTGCACAGAAATTTACTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTTATTATGAATACGCGCAACGCGTCTATTGTGGAGAAGGCGAAA > NZ_CP009273/673518‑673675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |