| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 937,171 | 0 | A | G | 66.7% | 0.8 / 10.3 | 15 | T253A (ACT→GCT) | dmsA | dimethylsulfoxide reductase subunit A |
| Reads supporting (aligned to +/- strand): ref base A (0/5); new base G (10/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGA > NZ_CP009273/937088‑937259 | gCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTACt > 2:440668/1‑90 (MQ=255) ccGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTAt > 1:298128/1‑90 (MQ=255) gTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGGTTTCTATCt > 2:268405/1‑90 (MQ=255) gTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATTTTTTACAGGCACGCCCGa > 1:261467/1‑90 (MQ=255) tAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGaa < 1:363336/90‑1 (MQ=255) gTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAAGCACGCCCGAAAACTAAt > 2:119473/1‑90 (MQ=255) cTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTTTTTTCTTTAAAAAGCACCCCCGAAAAATAATGGCCGc > 2:399078/1‑90 (MQ=255) tGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCa < 1:254215/90‑1 (MQ=255) ttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTATCTTTAACAAGCACGCCCGAAAACTAATGGCCGCCTGa > 2:65560/1‑90 (MQ=255) aaCCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTATCTTGAACAGGCACGCCAGAAAACTAATGCCCGCCTGatcatcatc > 1:371074/1‑90 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCGTCGATc < 1:268405/90‑1 (MQ=255) tGGCGAAACGCGAATGGGTGGCGGGGGGGGGGCTTACTATTTTTAAAAGGCACGCCCGAAAACTAATGGCCGCCTGATCATCATCGATcc > 2:356695/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAGGCACGCCCGAAAACTAATGCCCGCATGATCATCATCGATcc > 1:458793/1‑90 (MQ=255) aaaCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCt < 2:141237/90‑1 (MQ=255) gACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGa < 1:356695/90‑1 (MQ=255) | GCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGA > NZ_CP009273/937088‑937259 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |