| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,673,561 | 0 | A | C | 56.2% | 7.6 / 12.9 | 16 | Y293S (TAC→TCC) | ydgH | DUF1471 family protein YdgH |
| Reads supporting (aligned to +/- strand): ref base A (4/3); new base C (0/9); total (4/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAA > NZ_CP009273/1673493‑1673648 | cTACGGAAACATGCCCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAAAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCa < 1:475943/90‑1 (MQ=255) aaCATGACCGAATTCTCCTATCAGGTTGCAAAACGGGCCGGGAAAAAGGGTGCTAATTTTTCCCACACCCCCCGCCAGTGGCAGGAACGt < 2:470997/90‑1 (MQ=255) aaCATGACCGAAGTCCCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGt < 1:235836/90‑1 (MQ=255) aaCAGGCCCGAAGTCTCCTATCGGGTTGCGAACCGTCCCGCGAAAAAAGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGt < 2:259454/90‑1 (MQ=255) agacAGGTTGCGAAACGGGCCGCGAAGAAAGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc < 2:104325/87‑1 (MQ=255) ggTTGCAAAACGTGCCGAAAAAAAAGGTGCTAAGTTTTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc < 1:278803/90‑1 (MQ=255) tGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGa > 2:153696/1‑90 (MQ=255) aCGTGCCGGACAGAAAGGTGCAAAGTTTTCCCACTTCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGta < 1:405107/90‑1 (MQ=255) cgcaAAAAAGGGTGCTAATTTTTCCCCCACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTataaata < 2:148753/90‑1 (MQ=255) agaaAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTAt < 1:153696/90‑1 (MQ=255) gaaAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc < 1:247444/64‑1 (MQ=255) gaaAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc > 2:247444/1‑64 (MQ=255) aGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAg > 1:148753/1‑90 (MQ=255) gTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGtt < 2:292333/90‑1 (MQ=255) gCTAAGTTTTCCCCCCCCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTAc < 2:430328/90‑1 (MQ=255) ttACCACATCACCCGCCAGTGGAAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATaa > 1:293006/1‑90 (MQ=255) | CTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAA > NZ_CP009273/1673493‑1673648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |