Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,819,3180GA53.8% ‑0.2 / 11.3 13intergenic (‑124/+79)ves/spyenvironmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy
Reads supporting (aligned to +/- strand):  ref base G (5/1);  new base A (0/7);  total (5/8)
Fisher's exact test for biased strand distribution p-value = 4.66e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC  >  NZ_CP009273/1819243‑1819375
                                                                           |                                                         
gATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATcc                                             >  2:40686/1‑90 (MQ=255)
    gTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTc                                         >  2:228713/1‑90 (MQ=255)
     ttATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTcc                                        <  1:64202/90‑1 (MQ=255)
     ttATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAGCCGCCGATCCTGTcc                                        <  1:60948/90‑1 (MQ=255)
        tCGGCACCTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCAcc                                     <  1:225447/90‑1 (MQ=255)
             aaCTGGGGTGTTTTTTGTTAGGGGTGATCGGTTTTTTTTTACAATTTTTTGAGAAGAGAAAAAAAACCCGCCGATCCTGTCCACCGCAtt                                <  1:462137/90‑1 (MQ=255)
             aaCTGGGGTGTTTTACGTTAGGGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAtt                                <  1:309944/90‑1 (MQ=255)
             aaCTGGGGTGATTTCCGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCAtt                                <  1:249796/90‑1 (MQ=255)
                  gggTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGc                           <  1:107775/90‑1 (MQ=255)
                                 gtggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAGAGAAAACGGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg            >  2:419494/1‑90 (MQ=255)
                                       aTCAGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc      <  1:203309/90‑1 (MQ=255)
                                        tCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg     >  1:297762/1‑90 (MQ=255)
                                           ggCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgggc  >  2:260068/1‑88 (MQ=255)
                                                                           |                                                         
GATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC  >  NZ_CP009273/1819243‑1819375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑