| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,140,054 | 0 | C | T | 48.0% | 12.1 / 10.3 | 25 | A15T (GCA→ACA) | yghW | DUF2623 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (10/3); new base T (0/12); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTT > NZ_CP009273/3139978‑3140139 | gAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGcc > 2:217001/1‑90 (MQ=255) aGCCTAATACAAACCCGCTTTTATAATCGGCACAGATTTTTGTCACATTACCCGCACTGTCGGCATGCGTTGTTTTTAATCCCGCCATTa < 2:421220/90‑1 (MQ=255) gCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTaa > 1:237685/1‑90 (MQ=255) aaaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCa > 1:13175/1‑90 (MQ=255) aaaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCa > 1:310178/1‑90 (MQ=255) aaaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCa > 1:3321/1‑90 (MQ=255) aaaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCa > 2:301313/1‑90 (MQ=255) cgcgTTTATAACCGGCAAAAAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGTTTTTAATCCCGCCATTAAGCCTTTACCAAAAt < 1:455937/90‑1 (MQ=255) gcgTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATg > 2:452200/1‑90 (MQ=255) taATGGGCACAGAATTTTGTCACATTACCCCCACTGTCGGCATGCGTTGTTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTc < 1:322694/90‑1 (MQ=255) aTCGGCAAAAATTTTTGCCACATTACCCCCCCTGTCGGCATGCGTTGTTTTTAACCCCGCCATTAAGCCTTTACCAAAATGGTTATTCAt < 2:121817/90‑1 (MQ=255) gCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGtt > 2:389799/1‑90 (MQ=255) cacaGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTc < 1:301313/90‑1 (MQ=255) ttttGTCACATTACCCCCATTTTCGTCATGCTTTGTTTTTAACCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCAt < 2:237685/90‑1 (MQ=255) tttGTCACATTACCCGCACTGTCGGCATGCTTTTTTTTTAACCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCAtt < 2:13175/90‑1 (MQ=255) tttGTCACATTACCCGCACTGTCGGCATGCGTTTTTTTTAACCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCAtt < 2:73773/90‑1 (MQ=255) tttGTCACATTAACCGCACTGTCGGCATGCGTTGTTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCAtt < 1:452200/90‑1 (MQ=255) tttGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCAtt < 1:389799/90‑1 (MQ=255) tttGTAACATAACCCGCACTGTGGGCTTGCGTTTTTTTTACCCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCAtt < 2:310178/90‑1 (MQ=255) gccacaTTACCCGCCCTGTCGGCATGCGTTGTTTTTAACCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGc < 1:315202/88‑1 (MQ=255) aTTACCCGCCCTGTCGGCATGCTTTTTTTTTAACCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCtatat < 2:328577/90‑1 (MQ=255) aTTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCtatat < 1:335614/90‑1 (MQ=255) tacccGCCCTGTCGGCATGCGTTGTTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTAACCTCATTCGCTatatat < 1:245646/87‑1 (MQ=255) aCTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCata > 1:220656/1‑90 (MQ=255) gTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATAttt > 2:57019/1‑90 (MQ=255) | GAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTT > NZ_CP009273/3139978‑3140139 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |