| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,254,414 | 0 | A | C | 53.3% | 7.9 / 16.4 | 15 | V455G (GTG→GGG) | tdcE | 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base C (0/8); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCA > NZ_CP009273/3254328‑3254495 | ggCTGTCCATCCTTTTGTCGTAACCCACCACTTCGCCCACCGGGGGTGCTTTTTTCGCCCCGCCCTAACTCTTCAGCTTCTCGTcccccc < 2:485015/90‑1 (MQ=255) gCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAcccc > 1:265531/1‑90 (MQ=255) gTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccgcc > 1:202150/1‑90 (MQ=255) ttGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCg > 2:116825/1‑90 (MQ=255) ttGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCg > 2:195832/1‑90 (MQ=255) gtcgtcCATCGGGGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGc < 1:435662/90‑1 (MQ=255) gtcgtcCATCAGCGGTGTTTTTTTCGCCCCGCCCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGc < 1:237264/90‑1 (MQ=255) gtcgccCACCGGGGGTGTTTTTTTCGGCCCGACCTAATTCTTCACCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGc < 2:205897/90‑1 (MQ=255) tcgtcCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGcc > 1:205897/1‑90 (MQ=255) ggTGCTGTTTTCGCCCCGACCTAAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGt < 1:120310/90‑1 (MQ=255) tttcCGCCCCGCCCTAATTTTTCACCTTCCCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaa < 2:180347/90‑1 (MQ=21) aaTCTCCAGCTTCTCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCtt < 2:433126/90‑1 (MQ=37) cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAAt > 2:393346/1‑90 (MQ=255) aGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATc < 1:73331/90‑1 (MQ=255) aGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATc < 2:127499/90‑1 (MQ=255) tctcGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCa > 2:425631/1‑90 (MQ=255) | GGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCA > NZ_CP009273/3254328‑3254495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |