| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,679,381 | 0 | A | C | 52.0% | 18.1 / 34.0 | 25 | G1051G (GGT→GGG) | bcsC | cellulose synthase complex outer membrane protein BcsC |
| Reads supporting (aligned to +/- strand): ref base A (8/4); new base C (0/13); total (8/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-06 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAC > NZ_CP009273/3679293‑3679466 | cttcttCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCAc < 1:124067/90‑1 (MQ=255) tcCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGc > 1:384464/1‑90 (MQ=255) gCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTccc > 1:28384/1‑90 (MQ=255) gCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTccc > 2:52350/1‑90 (MQ=255) gCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTccc > 1:429173/1‑90 (MQ=255) aGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACg < 1:356354/90‑1 (MQ=255) ggtcggGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTAAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCa < 2:74361/90‑1 (MQ=255) aTTCATCAGCGGATAACGCGGCATGGTTTTGGTTCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTgcg < 2:153737/90‑1 (MQ=255) catcaGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTc > 1:307445/1‑90 (MQ=255) cGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCCCCCAGCCCCCACGCCCATTTTTCCGTGCGCTCCCGCca < 1:140430/90‑1 (MQ=255) cgcgGCTTGTTTTTGGTGGGTGAAGGCGCCCCCGAGCCAGCCGCCCCCAGCCCCCACGACCATTTTCCCGTGCGCCCCCGCCACATCAcc < 2:179611/90‑1 (MQ=255) gcttGGTTTTGGTGCGTGAAGGCCCCCCCGACCCAGCCGCCCCCACCCCCCCCGCCCATTTTTCCGTCCCCCCCCGCCACATCACCGGTa < 2:429173/87‑1 (MQ=255) ttttGGGGCGTAACTGCGCCCCCGGCCCAGCCCCCCCCACCCCCCACGACCAGTTTCCCGGGCGCTCCCGCCACATCACCGGTATGGCaa < 2:322812/90‑1 (MQ=255) tttgGGTGCGTAAATGCCCCCCCGCCCCAGCCCCCCCCCCCCCCCACCACCTTTTTCCCGTGCCCCCCCGCCACATCACCGGTATGGCaa < 2:424841/90‑1 (MQ=255) ggTGCGTAAAGGCCCCCCCGACCCAGCCCCCCCCCCCCCCCACGCCCATTTTCCCGGCCCCCCCCGCCACATCACCGGTATGGCAAACGa < 2:28384/90‑1 (MQ=255) tgggTGAATGCGCCCACGACCCAGCCGCCCCCAGCCCCCACGCCCTTTTTCCGGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACa < 1:356185/87‑1 (MQ=255) tGCGTGAATGCGCCCACGAGCCAGCCGCCCCCACCCCCCACGCCCATTTTTCCGTGCGCCCCCGCCACATCACCGGTATGGCAAACGACa < 2:202654/90‑1 (MQ=255) tGCGTGAATGCGCCCACGAGCCAGACGCCCCCACCCCCCACGCCCATTTTTCCGTGCCCTCCCGCCACATCACCGGTATGGCAAACGACa < 1:52350/90‑1 (MQ=255) gTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGt > 1:97379/1‑90 (MQ=255) gTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGt > 1:335930/1‑90 (MQ=255) ccACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCgg > 1:440127/1‑90 (MQ=255) ccACGACCCAGCCCCCCCCCGCCCCCCCGCCCTTTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCgg < 2:278796/90‑1 (MQ=255) ccGACCCAGCCCCCCCCCGCCCCCCCCACCATTTTCCCGGCCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGAc < 2:307445/89‑1 (MQ=255) ccGCCCCCAGCCCCCACCCCCTTTTTTCCGCGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAc < 2:243576/89‑1 (MQ=255) ccGCCCCCACCCCCCCCGACCATTTTTCCGTGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAc < 2:149561/89‑1 (MQ=255) | CTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAC > NZ_CP009273/3679293‑3679466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |