| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,086,494 | 0 | T | G | 60.0% | ‑1.1 / 13.6 | 15 | Y102S (TAC→TCC) | rhaM | L‑rhamnose mutarotase |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (9/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.28e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAA > NZ_CP009273/4086418‑4086583 | tatGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAAcatcttt > 2:379993/1‑89 (MQ=255) gTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAg > 1:211924/1‑90 (MQ=255) tttCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAg < 2:471560/82‑1 (MQ=255) tttCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAAAACTTCTTGCAg > 1:471560/1‑82 (MQ=255) tgtgCCGGAGGCTTCTCTGGCACATTGGGCAATTACGGCAGGGAAAACACTTCTTGCACCCCGCTACTCACCCGGGTGTTATCCGGGTTa > 1:185787/1‑90 (MQ=255) tgtgCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCCCTACTCACCCGGCTGTTATCCGGGGTa > 1:313294/1‑90 (MQ=255) ggAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCGCTACTCACCCGGCTGTTATCCGGGTTAGCGGGc > 2:28354/1‑90 (MQ=255) aGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCAt < 1:29739/90‑1 (MQ=255) ggCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATa < 2:299050/90‑1 (MQ=255) agctGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATc < 2:403779/88‑1 (MQ=255) caTTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCGCTACTCACCCGGCTGTTTTTCGGGGTAGCGGGGATAACATCGGTCatat > 2:80246/1‑90 (MQ=255) aTTGGGCAATTACGGCAGGGGAAAAACGTCTTTCAGCCCGCGACTCACCCGGCTGGTATCCGGGGTAGCGGGCGTAACATCGGTCatata > 2:342311/1‑90 (MQ=255) aTTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCGCTACTCACCCGGCTGTTTTCCGGGGTTGCGGGGCTAAAATCGGGCatata > 2:135377/1‑90 (MQ=255) gggCAATTACGGCAGGGAAAACACTTCTTTCAGCTCGCCACTCACCCGGCTGTTATCCGGGGTAGCGGGCATAAAATCGGTCatatatct > 1:403779/1‑88 (MQ=255) ttACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTccacca < 2:463643/90‑1 (MQ=255) tAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCaa < 1:80246/90‑1 (MQ=255) | TATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAA > NZ_CP009273/4086418‑4086583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |