| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,101,680 | 0 | T | C | 52.6% | 13.1 / 23.7 | 19 | K155E (AAA→GAA) | yiiQ | YiiQ family protein |
| Reads supporting (aligned to +/- strand): ref base T (7/2); new base C (0/10); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGC > NZ_CP009273/4101600‑4101761 | gAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGt > 2:247856/1‑90 (MQ=255) cccccTTTCCGCCGTTGCCCGCAACAACAAAACGCAGTCCCCCTTCTTTCTCGGTGTAATAACTTTTTTTTTTCCCCGCCGTTAGTAGCg < 1:95532/89‑1 (MQ=255) ttccgcgGTTGCCCGCAAAAAAAAAACGCGGTCCTCCTTCGTTCTGGGTGTAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCa < 2:479274/87‑1 (MQ=255) ccGCCGTTGCCCGCAAAAACATACCGCGGTCCCCTTTCTGTCGGGGTTAAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGt < 1:247856/89‑1 (MQ=255) ccGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGc > 2:299273/1‑90 (MQ=255) aTACCGCAGTGCCCCTTCTGTCTGGGTTTAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGtttt < 1:115872/90‑1 (MQ=255) cggggTGCCCCTTCTGTCTCGGTTAAATTCTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTc < 2:324486/86‑1 (MQ=255) cGCAGTGCCCCTTTTTTCTGGGTTTAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTc < 2:79026/90‑1 (MQ=255) cGCAGTCCCCTTTCTGTCTGGGTGAAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTc < 1:148805/90‑1 (MQ=255) gTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTa > 1:62242/1‑90 (MQ=255) gTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTa > 1:63519/1‑90 (MQ=255) ttCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt > 2:410456/1‑90 (MQ=255) tctgtctgGGTATAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTg < 1:419510/90‑1 (MQ=255) tGTCTGGGTTTAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAg < 2:62242/90‑1 (MQ=255) ttttgtttTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTc < 1:97914/58‑1 (MQ=255) gtttttttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTc < 1:293690/59‑1 (MQ=255) gtttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTc > 2:293690/1‑59 (MQ=255) gtttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTc > 2:97914/1‑59 (MQ=255) ttttttttCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGc < 1:242447/90‑1 (MQ=255) | GAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGC > NZ_CP009273/4101600‑4101761 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |