| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,464,532 | 0 | T | G | 52.6% | 16.5 / 17.9 | 19 | *198G (TGA→GGA) | bdcR | TetR/AcrR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (5/4); new base G (10/0); total (15/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTG > NZ_CP009273/4464460‑4464620 | gggCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGAccc > 1:209001/1‑90 (MQ=255) aCTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGAGATAAAAACTATTCTTGGGGGGGGATGCGGGCCTTGACCCGaaa > 2:209145/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTTTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAgcgc > 2:92918/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTTAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAacgc > 1:375412/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAacgc > 2:28154/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAAGCGGGAAAacgc > 2:193269/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAgcgc > 1:103296/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGAAAgcgc > 1:217227/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTTTTGAGGAGTGATGCGGGCCTTGACCCGAAAAGCGGGGAAacgc > 2:5712/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAacgc > 2:150021/1‑90 (MQ=255) gtgCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGGGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAGGGCCCGcc > 2:369749/1‑90 (MQ=255) gCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTc > 1:399979/1‑90 (MQ=255) aaCTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTa < 1:28154/90‑1 (MQ=255) tATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAgg < 2:45129/90‑1 (MQ=255) aTTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGc > 2:300829/1‑90 (MQ=255) ttGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGc < 1:369749/90‑1 (MQ=255) tGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATaa < 1:345425/62‑1 (MQ=255) tGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATaa > 2:345425/1‑62 (MQ=255) gTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTg > 2:128968/1‑90 (MQ=255) | GGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTG > NZ_CP009273/4464460‑4464620 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |