Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273337,5510CG53.8% 6.9 / 15.3 13A239G (GCG→GGG) yahJamidohydrolase family protein
Reads supporting (aligned to +/- strand):  ref base C (1/5);  new base G (7/0);  total (8/5)
Fisher's exact test for biased strand distribution p-value = 4.66e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTACGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTC  >  NZ_CP009273/337470‑337621
                                                                                 |                                                                      
gtgAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGGGCATTACg                                                                >  1:127190/1‑90 (MQ=255)
  gAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTTCgac                                                              >  1:441823/1‑88 (MQ=255)
        gtcgCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGGGCATTACGTCggcggc                                                        >  2:93993/1‑90 (MQ=255)
            ccTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTACGTCGGCGGCCTgg                                                    <  1:123432/90‑1 (MQ=255)
                    cagcaCGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTACGTCGGCGGCCTGGACCCGAcc                                            <  2:202556/90‑1 (MQ=255)
                     agcaCGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTACGTCGGCGGCCTGGACCCGACCa                                           <  1:212135/90‑1 (MQ=255)
                       caCGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGGGCATTACGTCGGCGGCCTGGGCCCGACCAgg                                         >  1:478231/1‑89 (MQ=255)
                                   ctgTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGGGCATTACGTCGGCGGGCCGGGCCCGCCCCGGGTTTGTGGggcg                             >  2:457113/1‑90 (MQ=255)
                                     gTCGAAATCTGAACCTTTAATGTGTGAAGCGATGCAGGCGGGGGGGCATTACGGCGGGGGCCTGGACCCCACCCGGGTTTGTGGggcggt                           >  1:288255/1‑88 (MQ=255)
                                     gTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGGGCATTTCGTCGGCGGCCCGGACCCGCCCAGGGTTGATGGggcggt                           >  1:435075/1‑88 (MQ=255)
                                                         tGCGTGAAGCGATGCAGGCGGGGGCGCATTACGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCa       <  1:151664/90‑1 (MQ=255)
                                                            gTGAAGCGATGCAGGCGGGGGGGCATTACGTCGGCGGCCTGGACCCCACCAGGGTTGATGGCGCGGTGGAAAAAACCCCCCACACCCTGt    >  2:151664/1‑90 (MQ=255)
                                                              gAAGCGATGCAGGCGGGGGCGCATTACGTCGGCGGCCTGGCCCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTc  <  2:338989/90‑1 (MQ=255)
                                                                                 |                                                                      
GTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTACGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTC  >  NZ_CP009273/337470‑337621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑