Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273645,9100TG53.8% 9.9 / 13.9 13T13P (ACC→CCC) citEcitrate (pro‑3S)‑lyase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/6);  new base G (7/0);  total (7/6)
Fisher's exact test for biased strand distribution p-value = 5.83e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGC  >  NZ_CP009273/645848‑645990
                                                              |                                                                                
gATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGTTGTTTCAGCGGAGc                                                       >  2:199345/1‑90 (MQ=255)
         gTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGTTGTTTGAGCGAAGCGGGAAACAt                                              >  2:270307/1‑90 (MQ=255)
         gTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTGCAGCGAAGCGGGAAACAt                                              >  1:139504/1‑90 (MQ=255)
              tGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCa                                         <  1:444395/90‑1 (MQ=255)
                    tCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGGTTTGGCAGCGAAACGGAAAACAATGGCAAacctc                                   >  1:469753/1‑90 (MQ=255)
                      gcgGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTccc                                 <  1:81248/90‑1 (MQ=255)
                      gcgGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTGCAGCGAAGCGGGAATCATTTGCAATCCTccc                                 >  1:133530/1‑90 (MQ=255)
                                    aGGCACAAACAACATGCTGCGGCGGGGGGGAGGTTTTCGTTTTTGGAGCGAAGCGGGAAACATTGGCAAACCTCCCCTGGCAGAGCCggg                   >  1:330890/1‑90 (MQ=255)
                                      gCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGAt                 <  2:283325/90‑1 (MQ=255)
                                            acaacaTGCTGCGGCGGGTGCGTGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCt           <  2:326545/90‑1 (MQ=255)
                                            acaacaTGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCt           <  2:362559/90‑1 (MQ=255)
                                                 aTGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCACATGGCAGAGCCGGGATACCGCTGgcgc      <  2:133530/90‑1 (MQ=255)
                                                     tgcGGCGGGGGCGGGGTTTTCGTTGTTGTAGCCGAACGGAAAAAATTGGCAATCCTCCCCTGGCAGAGGCGGGGTAGCGCCGGCGCGGGc  >  1:362559/1‑90 (MQ=255)
                                                              |                                                                                
GATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGC  >  NZ_CP009273/645848‑645990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑