| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,458,295 | T→G | I285S (ATC→AGC) | mgtA → | magnesium‑translocating P‑type ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,458,295 | 0 | T | G | 94.4% | 50.5 / ‑3.5 | 18 | I285S (ATC→AGC) | mgtA | magnesium‑translocating P‑type ATPase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (9/8); total (9/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01 | |||||||||||
CTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTT > NZ_CP009273/4458211‑4458366 | cTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGGGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGcc > 2:452320/1‑90 (MQ=255) aCAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCcgc < 1:51258/90‑1 (MQ=255) aGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGt < 1:108059/90‑1 (MQ=255) tGGTTTGGTCAACTGGCGGGGCGGGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGGGCAGCCGCGTCAGTATGctgctg > 1:398167/1‑90 (MQ=255) tttGGTCAACTGGAGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGAtt < 1:151692/90‑1 (MQ=255) gTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCt > 1:258165/1‑90 (MQ=255) tGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGc < 2:64141/90‑1 (MQ=255) gtgtTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATgg > 2:384267/1‑90 (MQ=255) gtgtTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATgg > 1:172625/1‑90 (MQ=255) gtTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCa > 1:175675/1‑90 (MQ=255) gtTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCa > 2:166858/1‑90 (MQ=255) aGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGc < 1:80278/90‑1 (MQ=255) aaaGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGt < 2:258165/90‑1 (MQ=255) aGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTa < 1:384267/90‑1 (MQ=255) gCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTaa > 2:30570/1‑90 (MQ=255) gAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATc > 1:275864/1‑90 (MQ=255) aGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCa < 2:398167/90‑1 (MQ=255) aTGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGtt < 1:452320/90‑1 (MQ=255) | CTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTT > NZ_CP009273/4458211‑4458366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |