| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 453,640 | 0 | T | G | 53.3% | 6.1 / 11.4 | 15 | G253G (GGT→GGG) | clpX | ATP‑dependent protease ATP‑binding subunit ClpX |
| Reads supporting (aligned to +/- strand): ref base T (4/3); new base G (8/0); total (12/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGA > NZ_CP009273/453555‑453729 | aaGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGGCTgg > 2:376892/1‑90 (MQ=255) gTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGa < 1:225222/90‑1 (MQ=255) tCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGGCTGGGTAAAATGATTTtccc > 2:194339/1‑89 (MQ=255) agGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGGGCGTTTGCCGGGCTGGGTAAAGTGATTTTCCACCgtgttg > 1:572089/1‑88 (MQ=255) ttGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGGCTGGGTAAAGTGATTTCCCACCGTGTAGAAAACGGc > 2:341724/1‑90 (MQ=255) tctAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGGCTGGATAAAGTGATTTCCCACCGTGTAGAAAACGGCTCCGGCATTGGtttt > 1:249960/1‑90 (MQ=255) ccTGTTTATTTGTGGCGGTGCGTTTGCCGGGCTGGGTAAAGGGATTTCCCACCGTGGAGAAACCGGCTCCCGCATTGGTTTTGGcgcggg > 2:400953/1‑88 (MQ=255) ccTGTTTATTTGTGGCGGTGCGTTTGCCGGGCTGGATAAAGGGATTTCCCCCCGTGTAGAAACCGGCTCCGGGATTTGGTTTGGggcggc > 2:405195/1‑88 (MQ=255) tttGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAg > 2:952/1‑90 (MQ=255) tgtgGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCAc < 1:454276/42‑1 (MQ=255) tgtgGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCAc > 2:454276/1‑42 (MQ=255) gtgGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGa < 1:194339/90‑1 (MQ=255) ggCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAg > 2:221871/1‑90 (MQ=255) ggCGGTGCGTTTGCCGGGCTGGATAAAGTGATTTCCCCCCGTGTAGAAACCGGCTCCGGCCTTGGTTTTTGGGCGGCGGGAAAAGCGAAg > 1:214390/1‑90 (MQ=255) gTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCg > 2:172275/1‑90 (MQ=255) tCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGa < 2:572089/90‑1 (MQ=255) | AAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGA > NZ_CP009273/453555‑453729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |