Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273760,1810GC58.3% 4.0 / 13.6 12P182P (CCG→CCC) sucDsuccinate‑‑CoA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/5);  new base C (7/0);  total (7/5)
Fisher's exact test for biased strand distribution p-value = 1.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGAT‑CCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGG  >  NZ_CP009273/760118‑760270
                                                               |                                                                                          
ggTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTTACCCCAT‑CCCCGGCTCTTACTTTTTTGACAt                                                                 >  2:411497/1‑90 (MQ=255)
ggTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGAT‑CCCGGGCTCTAACTTTATCGACAt                                                                 <  1:292138/90‑1 (MQ=255)
         gACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCCAT‑CCCCGGCTCTAACTTTTTTGACATTTTCGGAAt                                                        >  2:228314/1‑90 (MQ=255)
           ccACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGACCCCCTCCCCGGGCCCTAATTTTTTCG‑CAATCTTCAAATGt                                                      >  1:513658/1‑90 (MQ=255)
                                 gTCGACCTGTGTCGGTATCGGCGGTGACCCGAT‑CCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGa                                <  2:356832/90‑1 (MQ=255)
                                 gTCGACCTGTGTCGGTATCGGCGGGGACCCCAT‑CCCCGGCTCTTACTTTTTTGACATTTTCGAAAAGTTTGAAAAAAAACCCCAGACCGa                                >  1:21563/1‑90 (MQ=255)
                                   cGACCTGTGTCGGTATCGGCGGTGACCCGAT‑CCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAg                              <  1:552688/90‑1 (MQ=255)
                                               gTATCGGCGGGGACCCCAT‑CCCCGGCTCTAACTTTTTCGACATTCTCGAAATGTTTGAAAAAAAACCGCCGACCGAAGCGATCGtggtga                  >  1:183302/1‑90 (MQ=255)
                                                   cggcggGGACCCCAT‑CCCCGGCTCTTACTTTTTCGACATTTTCGGAATGTTTGAAAAAAAACCGCCGACCCAAGCGATCGTGATGATCgg              >  1:443902/1‑90 (MQ=255)
                                                        gTGACCCGAT‑CCCGGTCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTgaga         <  2:21563/90‑1 (MQ=255)
                                                        gTGACCCGAT‑CCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTgaga         <  2:541889/90‑1 (MQ=255)
                                                        gTGACCCCAT‑CCCCGGCTCTAACTTTATTGACATTCTCCAAAAGTTTGAAAAAAAACCGCCGACCGAAGCGATCGGGGTGATCGGTgaga         >  1:233208/1‑90 (MQ=255)
                                                               gAT‑CCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATcggcgg  >  1:427345/1‑90 (MQ=255)
                                                               |                                                                                          
GGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGAT‑CCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGG  >  NZ_CP009273/760118‑760270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑