Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273832,0480TG53.8% 7.9 / 11.2 13V81G (GTC→GGC) hcxBhydroxycarboxylate dehydrogenase HcXB
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base G (7/0);  total (9/4)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACG  >  NZ_CP009273/831977‑832131
                                                                       |                                                                                   
aCGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATcg                                                                   <  1:41426/90‑1 (MQ=255)
   cTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACGCCCGATGGCGATCgggc                                                                >  2:287024/1‑90 (MQ=255)
   cTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCCCGCTCGATGGCGGTCgcgc                                                                >  2:529065/1‑90 (MQ=255)
   cTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTc                                                                                    <  1:569669/70‑1 (MQ=255)
   cTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTc                                                                                    >  2:569669/1‑70 (MQ=255)
      cTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACGCCCGAAGGCGATCGCCCaat                                                             >  2:603574/1‑88 (MQ=255)
      cTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCAtt                                                             <  2:602777/90‑1 (MQ=255)
             cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGGGGGCACCCCCGATGGGGGTCGCGCCTTTTGGCAg                                                      >  2:405664/1‑90 (MQ=255)
               ggggCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCTCGATGGCGATCGCGCATTTGGGCAggg                                                    >  2:165560/1‑89 (MQ=255)
                               aaCCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGTCACGCTCGATGGGGATCGCGCATTTTGTCAGGGCGCGGCACATGAAGCg                                    >  2:114421/1‑90 (MQ=255)
                                  catcatGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCTCCATGGCGATCGCGCATTTTGTCAGGGCGCGGCACATGAAACGagg                                 >  2:506561/1‑88 (MQ=255)
                                                 gTTAAAGAGGCGGGGGGGGCGGGCACGCTCGATGGCGATCGCGCATTTTGTCAGGGCGGGGCACATGAAGCGGTGGCGGTGGGGGTTgag                  >  2:345168/1‑90 (MQ=255)
                                                                 cggcggTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACg  <  1:165560/90‑1 (MQ=255)
                                                                       |                                                                                   
ACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACG  >  NZ_CP009273/831977‑832131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑