| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,819,313 | 0 | G | A | 53.8% | ‑0.1 / 12.7 | 13 | intergenic (‑119/+84) | ves/spy | environmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy |
| Reads supporting (aligned to +/- strand): ref base G (5/1); new base A (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT > NZ_CP009273/1819238‑1819401 | cggTGGATGTTTATCGCCACCTGGGGTGATTTACGTTAGTGGTGATCAGTTTTTTTTTACAACTTTTTGAAAAGAAAAAAAAAAAccgcc < 1:132624/88‑1 (MQ=255)ccGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccgcc > 2:393106/1‑90 (MQ=255)ccGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccgcc > 2:48460/1‑90 (MQ=255) gATGTTTATCGGCACCTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACACCTTTTTGAAAAGAAAAAAAAAAACCGCCGATcc < 1:28224/90‑1 (MQ=255) gATGGTTATCGGCACCTGGGGTGTTTTACGTTAGGGGTGATCAGGTTTTTTTTACAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATcc < 1:106705/90‑1 (MQ=255) ttATGGGCACCTGGGGTGTTTTACGTTGGGGGTGATCGGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTcc < 1:198824/90‑1 (MQ=255) aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTa > 2:228986/1‑90 (MQ=255) gttaTTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 1:162536/87‑1 (MQ=255) gtgtTTTACTTTGGGGGTGATCAGTTTTTTTTTACACCTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 2:423054/90‑1 (MQ=255) gTGATTTACGTTAGGGGTGATCAGGCTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 2:51317/90‑1 (MQ=255) ttttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAtt > 1:468250/1‑56 (MQ=255) ttttttACAACTGTTTGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCAtt < 2:468250/56‑1 (MQ=255) ttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAGttt > 2:491100/1‑90 (MQ=255) agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGTCTGAAAGattatt > 1:328481/1‑90 (MQ=255) agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGGTTTAGTTTTTTTGGTGAAAGattatt > 1:578210/1‑90 (MQ=255) | CCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT > NZ_CP009273/1819238‑1819401 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |