| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,775,868 | 0 | A | C | 52.9% | 10.6 / 18.9 | 17 | V73G (GTA→GGA) | ypjA | adhesin‑like autotransporter YpjA/EhaD |
| Reads supporting (aligned to +/- strand): ref base A (7/1); new base C (0/9); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCT > NZ_CP009273/2775785‑2775954 | ttGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGc > 2:418333/1‑90 (MQ=255) tcccccACAAAATTGTTATGACTTCCTATATCTTGATACCCCCCAGATTCAATAAGACTCCCATTAGATCCCCCGCCATGAACATTctgc < 1:418333/88‑1 (MQ=255) gCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTctgc < 2:171776/89‑1 (MQ=255) gCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTctgct > 1:261298/1‑90 (MQ=255) gCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTctgc > 1:171776/1‑89 (MQ=255) cacaAAATTTTTATGACTTCCTATATCTTGATATCCCCCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGcc < 1:259066/90‑1 (MQ=255) aaaaTTTTTATCTCTTCCTATCTCTTTATATCCAACAAATTCAAAAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATg < 1:491517/90‑1 (MQ=255) aaTTTTTATGCCTCCCTATATCTTAATACCCACCAGATTAAAAAAGACTTCCATTAAATCCCCCGCCATGAACATTCTGCTGGCCATGGt < 1:310541/90‑1 (MQ=255) gTTATGACTTCCTATATCTTGATATCCCCCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGat < 2:261298/90‑1 (MQ=255) aCTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATg < 2:41523/90‑1 (MQ=255) atatCTTGATATCCCCCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTa < 2:429939/90‑1 (MQ=255) tGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATttgtt > 2:279383/1‑90 (MQ=255) gATATCCACCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATttgttg < 2:183606/90‑1 (MQ=255) cAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCa > 2:279725/1‑90 (MQ=255) tCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGtt > 2:179081/1‑90 (MQ=255) aGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATc > 2:497150/1‑90 (MQ=255) gATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCt < 1:279383/90‑1 (MQ=255) | TTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCT > NZ_CP009273/2775785‑2775954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |