Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,171,1070CA60.0% ‑1.3 / 10.2 10E55* (GAG→TAG) nudFADP‑ribose diphosphatase
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base A (0/6);  total (4/6)
Fisher's exact test for biased strand distribution p-value = 4.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCG  >  NZ_CP009273/3171037‑3171192
                                                                      |                                                                                     
aTCTGCTCAATCACCACACCTTCACCCCGCCCGGGGTCAAGGGGTAGCAAGACTGGGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCAcc                                                                    <  1:594346/90‑1 (MQ=255)
    gCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCAt                                                                >  2:144171/1‑90 (MQ=255)
                   ctccatcaCGCACTGGGTCAAAGGGTAGCAAGCCTGCGGCGTGCCCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGt                                                 <  1:386893/87‑1 (MQ=255)
                   cTTCACCACGCACTGGGTCAAAGGGAAGCAAGCCTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGt                                                 <  1:394949/90‑1 (MQ=255)
                     tcatcaCCCACTGGGTAAAAGGGTAGCAAGCCTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTg                                               <  2:335509/90‑1 (MQ=255)
                                      aaaGGGTAGAAAGACGGCGGCGTGCCCGCGCAAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaaa                              <  1:593078/90‑1 (MQ=255)
                                           gTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtat                         >  2:131686/1‑90 (MQ=255)
                                                 aGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGAt                   >  2:243198/1‑90 (MQ=255)
                                                   aCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCt                 >  2:263263/1‑90 (MQ=255)
                                                                  cgcTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcg  <  1:95239/90‑1 (MQ=255)
                                                                      |                                                                                     
ATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCG  >  NZ_CP009273/3171037‑3171192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑