| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,493,428 | 0 | T | G | 54.5% | 17.7 / 21.7 | 22 | L54V (TTA→GTA) | frlA | fructoselysine/psicoselysine transporter |
| Reads supporting (aligned to +/- strand): ref base T (3/7); new base G (12/0); total (15/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGAT > NZ_CP009273/3493345‑3493496 | ccGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGGTTAttt > 1:2219/1‑89 (MQ=255) ttttGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATccc > 2:197035/1‑90 (MQ=255) tGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCa < 1:535042/90‑1 (MQ=255) aTCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGGTAATTTTGATCCCCCCAAt > 2:569048/1‑90 (MQ=255) aTCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGGTAATTTTGATCCCCCAAAt > 2:136137/1‑90 (MQ=255) gtgGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGGTAATTGTGGTTCCCCAAAagtgg > 2:446538/1‑89 (MQ=255) ggTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTc < 1:542620/90‑1 (MQ=255) gTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGGTAATTTTGATCCCCCCAAAGTGGGTCt > 2:297146/1‑90 (MQ=255) aGTGGCAAAAGCAGCGGTCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGc < 1:210975/90‑1 (MQ=255) aGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGGTAATTTTGATCCCGCCAATTTGCGGCTTTGc > 2:542620/1‑90 (MQ=255) tGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGGTAATTGTGATCCCGCAAAAGTGGGGCTATGCgg > 2:82740/1‑90 (MQ=255) gCAAAAGCAGCGGTCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCCCCAAAGTGCGTCTTTGCggga > 2:210975/1‑88 (MQ=255) gCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGGTAATTTTGATCCCCCAAAAGTGCGGCTATGCggga > 2:83784/1‑88 (MQ=255) cGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATc < 2:144231/90‑1 (MQ=255) cGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGgcg < 1:82740/90‑1 (MQ=255) cGGTGCTCGCGTTTGTCATTGGCGGGGTAATTTTGATCCCCCCAAAGTGGGGCTTTTCGGGACTATCCACCCCTTTTCCCGGAAAAGgcg > 1:130738/1‑90 (MQ=255) ggTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGgcgc < 1:197035/90‑1 (MQ=255) gCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGa > 2:23382/1‑90 (MQ=255) gCTCGCGTTTGTCATTGGCGGGGTAATTTTGGTCCCCCAAATGTGCGTCTCTGCGGGACTATCCCCCGCTTTTCCGGGAAAAGGCGCAGa > 2:357896/1‑90 (MQ=255) gCTCGCGTTTGTCATTGGCGGGGTAATTTTGATCCCCCAAAAGTGCGGCTGTGCGGGACTATCCACCCCTTTTTCGGAAAAAAGCGCAGa > 1:155264/1‑90 (MQ=255) cTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGAt < 1:333478/90‑1 (MQ=255) cTCGCGTTTGTCATTGGCGGGGTAATTTTGATCCCCCCAATGTGCGGCTTTGCGGGACTATCCCCCCCTTTTCCCGGAAAAGGCGCAGAt > 1:115616/1‑90 (MQ=255) | CCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGAT > NZ_CP009273/3493345‑3493496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |