| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,623,890 | 0 | A | C | 53.3% | 7.8 / 12.9 | 15 | W25G (TGG→GGG) | yhiI | HlyD family efflux transporter periplasmic adaptor subunit |
| Reads supporting (aligned to +/- strand): ref base A (6/1); new base C (0/8); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTT > NZ_CP009273/3623805‑3623950 | ttGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCcaccac < 1:146671/90‑1 (MQ=255) ttCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGccgccac > 1:119733/1‑90 (MQ=255) cccGTCGCTTCAAGCCCCCCATTACTGACAGAAAACCCTCCCGGCACCCCTGCGGGGCGCAACAGCCCCCACGCCACGATAGccgccacc < 1:17962/89‑1 (MQ=255) gTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGccgccaccgcc > 1:122174/1‑90 (MQ=255) gTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGccgccaccgcc > 1:184541/1‑90 (MQ=255) gTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGccgccaccgcc > 2:149998/1‑90 (MQ=255) tCAATGCCCCCATTACTGACAGAAAACCCTCCCGCCCCCCCGGCCGGGCGCAACACCCCCCCCGCCACGATAGCCGCCACCGCCAGTAAc < 1:159552/90‑1 (MQ=255) ttACGGACAGAAAACCCTCCCGCCCCCCCGGCGGGGCCCAACACCCCCCCCGCCACGATAGCCCCCACCGCCAGTAACCCGACAACccac < 2:519804/90‑1 (MQ=255) aCTGACAGAAAAGCCTCCCGGCCCCCCTGCGGGGCGCAACAGCCCCCCCGCCACGATAGCCGCCACCGCCAGTAACCCGACAACccacca < 2:449424/90‑1 (MQ=255) gACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGc > 1:300997/1‑90 (MQ=255) gCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGa > 1:223153/1‑90 (MQ=255) aaccccTCCCGGCCCCCCGGCCGGGCCCAACACCCCCCCCCCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATg < 1:158357/86‑1 (MQ=255) aaccccTCCCGCCACCCCGCCCGGGCCCACCCCCCCCCCCGCCCCAATACCCGCCCCCGCCAGTAACCCGACAACCCACCACGCCAGATg < 2:298693/86‑1 (MQ=255) tcccGGCCCCCCGGCCGGGCCAAACACCCCCCCCCCCACGATACCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCtt < 2:349738/88‑1 (MQ=255) tcccGGCACCCCTGCGGGGCCCAACACCCCCCCCGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCtt < 2:1084/88‑1 (MQ=255) tcccGGCACCCCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCtt < 2:198362/88‑1 (MQ=255) | TTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTT > NZ_CP009273/3623805‑3623950 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |