| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,527,926 | 0 | A | C | 53.3% | 5.7 / 14.4 | 15 | G219G (GGT→GGG) | nanM | N‑acetylneuraminate epimerase |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base C (0/8); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACC‑TTTATT‑CACA‑ACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGA > NZ_CP009273/4527841‑4528012 | aaatattaCCGGTGAAAGCAAGTGCAAATACGGCATCAGCCCGCAATCCTGGTTTCGCTGCGCCATAAATAATCCAGGTTTTACAACC‑tt < 1:484648/87‑1 (MQ=255)aaTTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATGAGCCAGGTTTTATCACC‑tt < 1:60221/90‑1 (MQ=255) attaCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACC‑TTTAtt > 2:364237/1‑90 (MQ=255) tACGGCACCCGTTCGCAATCCTGTTTTGGCTTCGCCATTAATAAGCCAGTTTTTATCCCT‑TTTATT‑CACA‑ACCGCCGCACCAGCCGTTcc < 2:160641/90‑1 (MQ=255) cccTGTTTTGGTTTCGCCATTAATAAGCCAGGTTTTATCCCC‑TTTATT‑CACA‑ACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc < 2:163353/89‑1 (MQ=255) ccTTGTTTGGCCTCCCCCTTAAAAAACCCGGTTTTTACCCC‑TTTTTTCCAAA‑CCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc < 1:12309/90‑1 (MQ=255) ggTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACC‑TTTATT‑CACA‑ACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCg > 2:175419/1‑90 (MQ=255) tttGCCTTCCCCATAAATAAGCCAGGTTTTATCCCT‑TTTTTT‑CCCA‑ACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTa < 2:79985/90‑1 (MQ=255) tttGCCTCCCCCATAAAAAACCCGGTTTTTATCCCT‑TTTATT‑CACA‑ACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTa < 2:151334/90‑1 (MQ=255) ttGGCTTCCCCATTAAAAAGCCCGGTTTTTTCCCCTTTTACC‑CACC‑CCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTa < 1:271031/90‑1 (MQ=255) ggCTTCGCCATTAATAAGCCAGGTTTTATCACC‑TTTATT‑CACA‑ACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACt > 2:596647/1‑90 (MQ=255) cGCCATTAATAAGCCAGGTTTTATCACC‑TTTATT‑CACA‑ACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAtt > 2:148515/1‑90 (MQ=255) aTTAAAAAGCCGGTTTTTTCCCCC‑TTTATT‑CAAA‑CCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtg < 2:437568/90‑1 (MQ=255) gCCAGGTTTTTTCCCC‑TTTTTT‑CCCACCCCGCCGCCCCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAg < 1:7344/90‑1 (MQ=255) aTCACC‑TTTATT‑CACA‑ACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGa > 2:154552/1‑90 (MQ=255) | AATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACC‑TTTATT‑CACA‑ACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGA > NZ_CP009273/4527841‑4528012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |