| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,142,237 | 0 | T | G | 54.2% | 14.6 / 27.2 | 24 | T264P (ACC→CCC) | ppc | phosphoenolpyruvate carboxylase |
| Reads supporting (aligned to +/- strand): ref base T (1/10); new base G (13/0); total (14/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.61e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTG‑ACGTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGTAAAACGGACCGGAACAAATTCGACGGGCAG > NZ_CP009273/4142159‑4142321 | gTTCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGAAATTggggg > 2:193399/1‑87 (MQ=255) cAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGt < 1:577935/81‑1 (MQ=255) cAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGt > 2:577935/1‑81 (MQ=255) cAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGTG‑ACGTTTgg > 1:577527/1‑90 (MQ=255) tatCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTG‑ACGTTCGGGTTGCCGTcgc < 2:721145/90‑1 (MQ=255) tttCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGGG‑ACGTTTGGGGTGGCGCCGCgggc > 2:37224/1‑88 (MQ=255) cAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATTGGCAGGG‑GCGTTTGGGGTTCCCTCGCGGGcgcc > 2:662771/1‑90 (MQ=255) cAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGGGAACGTCCGGG‑TGCCggcgccggcgcc > 2:324530/1‑90 (MQ=255) cAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGGG‑AAGTTTGGGGTGCCGTCGCGGGcgcc > 1:494786/1‑90 (MQ=255) aCAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGTG‑ACGTTCGGGGTGCCGTCGCGGGCGccccccc > 1:331986/1‑89 (MQ=255) tCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTG‑ACGTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCAc < 1:570127/90‑1 (MQ=255) tCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTG‑ACGTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCAc < 2:577527/90‑1 (MQ=255) tCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTG‑ACGTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCAc < 1:266204/90‑1 (MQ=255) ggCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTG‑ACGTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGt < 2:54016/90‑1 (MQ=255) tttCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGTG‑ACGGTCGGGGTGCCGTCGCGGGCGCCGCCCCTCCCCGAAGTaaa > 1:334577/1‑90 (MQ=255) tttCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGGG‑GCGTTCGGGGTGCCGGCGCGGGCGCCGCCCCTCCACCAAGGaaa > 2:266204/1‑90 (MQ=255) ggCTGAGTAGCAGGACGTGGCGGGGGGTATCGGCAGTG‑TCGTTCGGGGTGGCGGCGGGGGGGGCGCCCGGCCCCGAGGGAAAAAGGGCCg > 2:455380/1‑90 (MQ=255) aGGACGTGGCGGGTGATATCGGCAGTG‑ACGTTCGGGTTGCCGTCGCGGGCGCCGCCCATCCACGAAGTAAAACGGACCGGAACAAATTCg > 1:191362/1‑90 (MQ=255) aCGTGGCGGGGGATATCGGCAGTG‑ACGTTCGGGGTGCCGTCGCGGGCGCCGCCCCTCCCCGAAGGAAAAAGGACCGGAACAAATTctgtc > 2:383307/1‑86 (MQ=255) tGGCGGGTGATATCGGCAGTG‑ACGTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGTAAAACGGACCGGAACAAATTCGACGGGc < 1:58395/90‑1 (MQ=255) tGGCGGGTGATATCGGCAGTG‑ACGTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGTAAAACGGACCGGAACAAATTCGACGGGc < 1:707716/90‑1 (MQ=255) gCGGGTGATATCGGCAGTG‑ACGTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGTAAAACGGACCGGAACAAATTCGACGGGCAg < 1:324530/90‑1 (MQ=255) gCGGGTGATATCGGCAGTG‑ACGTTCGGGTTGCCGCCGCGGTCGCCGCCCATCCACGAAATAAAACGGACCGGAACAAATTCGACGGGCAg < 1:37224/90‑1 (MQ=255) gCGGGGGATATCGGCAGTG‑ACGTTCGGGGTGCCGGCGCGGGCGCCCCCCCTCCCCGGAGGAAAAAGGGCCCGGAAAAATTCGACggggcg > 2:440605/1‑87 (MQ=255) | GTTCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTG‑ACGTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGTAAAACGGACCGGAACAAATTCGACGGGCAG > NZ_CP009273/4142159‑4142321 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |