| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 71,561 | 0 | T | G | 57.1% | 4.9 / 13.3 | 14 | N136T (AAC→ACC) | thiB | thiamine ABC transporter substrate binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (8/0); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGA > NZ_CP009273/71475‑71644 | gCCCCGGTGTACTGG‑GCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGttttt < 2:700295/90‑1 (MQ=255) ccccGGTGTACTGGTGCGCGGTTCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGttttt < 2:98938/90‑1 (MQ=255) cGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGgttttttt > 1:537559/1‑87 (MQ=255) aCTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGtttttt > 1:161593/1‑90 (MQ=255) gcgcGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTttttttttttt > 2:101698/1‑87 (MQ=255) gcgcGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTGTttttttt > 2:138942/1‑87 (MQ=255) tGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGAGGTTTTCAGTTTGTTCTTGTCATAAACGAAg > 1:587846/1‑90 (MQ=255) aTAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGc < 1:29428/90‑1 (MQ=255) cACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTTTTTTTTTTATAAAAGAAGGCGGAgga > 1:193547/1‑88 (MQ=255) ccGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGcc < 2:724004/90‑1 (MQ=255) tttGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCaa < 2:560294/90‑1 (MQ=255) tGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAAc < 1:20949/90‑1 (MQ=255) tGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTGGTTCCTGTCATAAACGAAGGCGAAGTAGCCATAATCAAAc < 1:482574/90‑1 (MQ=255) ctctCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTTTTTTAAAAAAAAGGGAAAAAGGCATAATAAAAAGGGACg > 2:224664/1‑90 (MQ=255) tttCAGGCTTTGTGGCGGGGTTTTTTTTTTTTTTTTTTTTTTAAAAAAGGCGGAGAAACCATAATTAAAAAGTACGCAAAAGTCgttatt > 2:614649/1‑90 (MQ=255) tttCAGGCTTTGTGGCGGGGTTTTTAGTTTGTTTTTTTCATAAAAGAAAGCGAAGAAGCCATAATCAAAAGGGACGAAAGAGTCattatt > 1:84053/1‑90 (MQ=255) ggCGGTTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGa < 2:235073/90‑1 (MQ=255) | GCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGA > NZ_CP009273/71475‑71644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |