| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,791 | G→A | intergenic (+160/‑32) | kefC → / → folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,791 | 0 | G | A | 86.7% | 80.8 / 4.5 | 30 | intergenic (+160/‑32) | kefC/folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base G (4/0); new base A (16/10); total (20/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.19e-01 | |||||||||||
GAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAA > NZ_CP009273/49706‑49873 | gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGc > 2:160978/1‑90 (MQ=255)gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGc > 1:716533/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg > 2:297398/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCg > 2:718626/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCg > 1:479696/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCACCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCg > 1:372146/1‑90 (MQ=255) gTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAAt > 1:682164/1‑90 (MQ=255) tCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAttttttt > 1:426001/1‑90 (MQ=255) cGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAAtttttttt > 2:443447/1‑90 (MQ=255) cagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCggg < 2:716533/90‑1 (MQ=255) gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGaa > 2:390493/1‑90 (MQ=255) ttttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGa < 1:718626/90‑1 (MQ=255) ttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAAt > 1:490332/1‑80 (MQ=255) ttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAAt < 2:490332/80‑1 (MQ=255) ttCCTCAACATCACCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc < 2:372146/90‑1 (MQ=255) tcatcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc > 1:298448/1‑80 (MQ=255) tcatcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc < 2:298448/80‑1 (MQ=255) atcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAAt > 2:475799/1‑68 (MQ=255) atcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAAt < 1:475799/68‑1 (MQ=255) cGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt > 2:673585/1‑90 (MQ=255) cGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt > 1:579717/1‑90 (MQ=255) gCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt > 1:564953/1‑89 (MQ=255) gCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt < 2:564953/89‑1 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGt > 1:40808/1‑90 (MQ=255) gTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTa > 1:85285/1‑90 (MQ=255) ttACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATgg > 2:414636/1‑90 (MQ=255) tACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGa > 2:326144/1‑90 (MQ=255) aCGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGaa < 2:126868/90‑1 (MQ=255) aCGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGaa < 1:35775/90‑1 (MQ=255) ataATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt < 1:62690/58‑1 (MQ=255) | GAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAA > NZ_CP009273/49706‑49873 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |