| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,458,295 | T→G | I285S (ATC→AGC) | mgtA → | magnesium‑translocating P‑type ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,458,295 | 0 | T | G | 93.9% | 96.5 / ‑1.5 | 33 | I285S (ATC→AGC) | mgtA | magnesium‑translocating P‑type ATPase |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base G (12/19); total (12/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01 | |||||||||||
TTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGAC > NZ_CP009273/4458208‑4458380 | ttGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCa < 2:541210/90‑1 (MQ=255) cTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGcc < 2:468157/90‑1 (MQ=255) cTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGcc < 1:171150/90‑1 (MQ=255) cTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGcc < 1:58287/90‑1 (MQ=255) aCAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCcgc > 1:387533/1‑90 (MQ=255) ggTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTc < 2:387533/90‑1 (MQ=255) ggTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTc > 1:429406/1‑90 (MQ=255) ggTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTc < 2:104868/90‑1 (MQ=255) ggTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTc < 1:580806/90‑1 (MQ=255) tGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAg > 2:485438/1‑90 (MQ=255) tGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAg < 2:27913/90‑1 (MQ=255) tGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAg > 1:627921/1‑90 (MQ=255) cAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGACCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTAt > 2:612784/1‑90 (MQ=255) aTACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTAtgc > 2:186833/1‑90 (MQ=255) tttGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGAtt < 1:556865/90‑1 (MQ=255) tttGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGAtt < 2:429406/90‑1 (MQ=255) ttGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTc > 2:196970/1‑90 (MQ=255) ggTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGAtt < 1:51795/87‑1 (MQ=255) ggTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGAtt > 2:51795/1‑87 (MQ=255) ggTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGc > 1:303076/1‑90 (MQ=255) ggTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGc > 2:569848/1‑90 (MQ=255) tCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCtt < 2:490035/90‑1 (MQ=255) cGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATg < 1:485438/90‑1 (MQ=255) cGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATg < 2:627921/90‑1 (MQ=255) gtTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCtt < 1:559291/74‑1 (MQ=255) gtTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCtt > 2:559291/1‑74 (MQ=255) aGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCggtgg < 1:316498/90‑1 (MQ=255) ggAAAGCGAGCCGAATGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCt > 1:508376/1‑90 (MQ=255) aTGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGtt < 2:463035/90‑1 (MQ=255) aTGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGtt < 1:357316/90‑1 (MQ=255) aTGCCTTTCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGtt < 2:709682/90‑1 (MQ=255) tCAGCAAGGGAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCaa < 1:569848/90‑1 (MQ=255) gggAGCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGAc < 2:391449/90‑1 (MQ=255) | TTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGAC > NZ_CP009273/4458208‑4458380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |