Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,152,7450AG57.1% 8.5 / 13.9 14G171G (GGA→GGG) ycfHmetal‑dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/6);  new base G (8/0);  total (8/6)
Fisher's exact test for biased strand distribution p-value = 3.33e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGAT  >  NZ_CP009273/1152675‑1152826
                                                                      |                                                                                 
cGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTGGGTTTCGGGTTTTTTATCTCCTTTTTCg                                                                >  2:206498/1‑90 (MQ=255)
 ggATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCgg                                                               <  1:206498/90‑1 (MQ=255)
    ttGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCAt                                                            <  1:572379/90‑1 (MQ=255)
                actacACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATGGGTTTCGGGTTTTAAATCTCCTTTTTCCGCCTTTTGACCCTCCg                                                >  2:86488/1‑90 (MQ=255)
                     acTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTAATTGATCTCGGGTTTTACATCTCCTTTTTCCGCATTGTGGCCTTTCGTAATg                                           >  1:217369/1‑90 (MQ=255)
                      cTGTTTTACAGAGGACAGAGAAACGGCGGGGGAATTACTGGATTTCGGGTTTTTAATTTCCTTTTTTGGCAATGTGAACTTCCGTTATGc                                          >  1:534765/1‑90 (MQ=255)
                        gTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGGTCTCGGGTTTTTTATTTCCTTTTTCGGCATTTTGGCCTTCCGTAAttctg                                        >  2:617379/1‑86 (MQ=255)
                        gTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGGTCTCGGGTTTTAAATCTCCTTTTTCGGCCTTGTGACCCTCCCTAATGCgg                                        >  2:23899/1‑90 (MQ=255)
                        gTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTTGATTTCGGGTTTTTTATTTCCCTTTTCCGCACTTTGACCCTTCCTAATGTgg                                        >  1:33528/1‑90 (MQ=255)
                                            aCGGCGGGGAAATTACTGGATCTCGGGTTTTAAATCTCCTTTTTCCGCATTGTGACCTTCCCTAATTCGGAGCAAATGCGCGATGCTgcg                    >  2:135819/1‑90 (MQ=255)
                                                    tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCTGAGCAACTGCGCGATGCTGCGCGTTATGt            <  1:617379/90‑1 (MQ=255)
                                                    tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt            <  1:23899/90‑1 (MQ=255)
                                                    tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt            <  2:260531/90‑1 (MQ=255)
                                                              gATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGAt  <  2:217369/90‑1 (MQ=255)
                                                                      |                                                                                 
CGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGAT  >  NZ_CP009273/1152675‑1152826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑