| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,548,312 | 0 | T | G | 53.3% | 7.4 / 15.5 | 15 | T539P (ACC→CCC) | maeA | malate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (8/0); total (11/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGGTACGGCGGTAGTCGCGGTATTCGGC‑TTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTT > NZ_CP009273/1548236‑1548395 | gAGGTACGGCGGTAGTCGCGGTATTCGGC‑TTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTCACCCCCACg > 2:28590/1‑90 (MQ=255)gAGGTACGGCGGTAGTCGCGGGATTTGGC‑TTGCCAGAAATTATCGTCAATGGGCTGATTGAGGGGTTCGGCAGAGGGTTTTACCGCCACg > 1:60878/1‑90 (MQ=255)gAGGTACGGCGGGAGTCGCGGGATTCGGG‑TTTCCAGAAAATATCGTCAATGGGCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCCCCCCg > 1:249453/1‑90 (MQ=255) aGGTACGGCGGTTGTGGCGGGAATCCGCTTTGCCGGAAA‑TATCCGCAATGGCCTGTTGCAGGGAGTCGGCAAAGGGTTTTACCGCCACGc > 2:528989/1‑90 (MQ=255) tACGGCGGTAGTCGCGGGATTCGGC‑TTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCGCCCCGCCtt > 1:165027/1‑90 (MQ=255) cggcggTAGTCGCGGTATTCGGC‑TTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCGCCACGCCTtgc > 2:128175/1‑90 (MQ=255) gTAGTCGCGGGATTTGGC‑TTGCCAGAAAATATCGTCAATGGCCTGTTGCAGGGGTTCGGCAGAGGGTTTTACCGCCACGCCCttctgctg > 1:140184/1‑90 (MQ=255) tAGTCGCGGGATTCGGC‑TTGCCCGAAAATATCGTCAATGGCCTGTTGCCGGGGGTCGGGGGGGGGTTTCCCCCCCCCGCCCttctgctgc > 1:320865/1‑90 (MQ=255) ccAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCaaa > 1:11053/1‑90 (MQ=255) aTTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAAt < 2:529933/90‑1 (MQ=255) gTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCt > 1:641285/1‑90 (MQ=255) gcggggTTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACATTCTGaa < 1:28590/87‑1 (MQ=255) ttCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCttt > 1:282928/1‑90 (MQ=255) tCGGCAGAGGTTCTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTc < 2:320865/90‑1 (MQ=255) cAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGtt < 2:641285/90‑1 (MQ=255) | GAGGTACGGCGGTAGTCGCGGTATTCGGC‑TTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTT > NZ_CP009273/1548236‑1548395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |