| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,401,195 | 0 | T | G | 56.2% | 9.3 / 16.4 | 16 | V77G (GTA→GGA) | panF | sodium/pantothenate symporter |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (9/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAC > NZ_CP009273/3401109‑3401280 | ggCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTATAAATACGGGCTGGGCTGGGGAtt > 2:13470/1‑90 (MQ=255) cTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGGATTGCTGGCgatg > 2:245106/1‑90 (MQ=255) gACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCt < 2:631120/90‑1 (MQ=255) tatCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGc < 1:588780/90‑1 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCCGGGGCTGCTTATAAAAACGGGCTGGGGTGGGGATTTGTGGGGGTGATTTAGCTTCCTTCCGTCt > 2:588780/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGGGGTGCTTTTAAATACGGGCTGGGGTGGGGATTTCTGGGGGTGATTTAGCTTCCTGCAGTCt > 2:601884/1‑90 (MQ=255) gCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTTTAAATACGGGCTGGGGTGGGGATTTCTGGGGGTGATTTAGCTTCCTTCAGTCTgg > 2:395463/1‑90 (MQ=255) gCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTgg > 2:106814/1‑90 (MQ=255) cAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCt > 2:38705/1‑90 (MQ=255) gTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCttt < 2:264977/90‑1 (MQ=255) gTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGGTGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACt > 2:612150/1‑90 (MQ=255) gTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACt > 2:189965/1‑90 (MQ=255) cggGCCAGGAGCTGCTTATAAATACCTGCTTGGTTGGGTATTGCTGGCGATCATTCACCTTCCTCCAGTCTGGCTTTCCCTCGGTATtct < 1:23441/90‑1 (MQ=255) gggctgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGcgccg > 2:282881/1‑90 (MQ=255) tgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAc < 1:13470/90‑1 (MQ=255) tgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAc < 1:565938/90‑1 (MQ=255) | GGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTAC > NZ_CP009273/3401109‑3401280 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |