| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,958 | 0 | G | A | 57.1% | ‑0.5 / 11.7 | 14 | L57L (CTG→TTG) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base G (5/1); new base A (0/8); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058888‑4059038 | atatTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGACATAg < 1:14020/90‑1 (MQ=255) tttCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGc > 1:414430/1‑90 (MQ=255) tttCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGc > 2:267982/1‑90 (MQ=255) gcgAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCa > 2:466258/1‑90 (MQ=255) gcgAGTCGAAAAAAACCCGGGGGACCATACGGGAAAACG‑GGAAAAAAACCTCCCCCCCAAAAAAAATAACCCCGCCATAGTAAGCAGGCa < 1:393634/90‑1 (MQ=255) aaaTCGGGTGAGCATATGGGAAACCGGGGAAAAAAACTTCGCCACA‑AAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 2:465568/90‑1 (MQ=255) tatCGGAAAACGCGGAAAAAAACTCCCCCCCC‑AAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTga < 2:67535/90‑1 (MQ=255) aaaCGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATa > 2:6699/1‑90 (MQ=255) aaCGCGGAAAAAACCTTCCCCACA‑AAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATAc < 2:187113/90‑1 (MQ=255) gcgGAAAAAACCTCCGCCACC‑AAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAGTTCATCGGTATAAAATTTGAGGAGATACaga < 2:401813/90‑1 (MQ=255) aaaaacCTCCCCCACA‑AAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTAcc < 1:466258/90‑1 (MQ=255) aaacCTTCGCCACA‑AAAAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGa < 2:431607/90‑1 (MQ=255) tcccccaca‑aAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCag < 1:197101/86‑1 (MQ=255) tCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCaga > 1:421864/1‑90 (MQ=255) | ATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058888‑4059038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |